Direct cooperation between androgen receptor and E2F1 reveals a common regulation mechanism for androgen-responsive genes in prostate cells

D. M. Altintas, M. S. Shukla, D. Goutte-Gattat, D. Angelov, J. P. Rouault, S. Dimitrov, Jacques Samarut

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have studied the regulation of ATAD2 gene expression by androgens in prostate cells. ATAD2 is a coactivator of the androgen receptor (AR) and the MYC protein. We showed that ATAD2 expression is directly regulated by AR via an AR binding sequence (ARBS) located in the distal enhancer of its regulatory region. The gene is also regulated by the E2F1 transcription factor. Using knockdown and chromatin immunoprecipitation technique approaches, we could demonstrate that AR and E2F1 functionally collaborate and physically interact between each other. From the analysis of chromatin conformation, we conclude that this cooperation results from a chromatin looping over the ATAD2 promoter region between the ARBS and E2F1 binding site in an androgen-dependent manner. Furthermore, we could show that several genes overexpressed in prostate cancer and potentially involved in several aspects of tumor development have an ARBS and an E2F1 binding site in their regulatory regions and exhibit the same mechanism of regulation by both transcription factors as ATAD2.

Original languageEnglish
Pages (from-to)1531-1541
Number of pages11
JournalMolecular Endocrinology
Volume26
Issue number9
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Fingerprint

Androgen Receptors
Androgens
Prostate
Nucleic Acid Regulatory Sequences
Genes
Chromatin
E2F1 Transcription Factor
Binding Sites
Chromatin Immunoprecipitation
Gene Expression Regulation
Genetic Promoter Regions
Prostatic Neoplasms
Transcription Factors
Neoplasms
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Direct cooperation between androgen receptor and E2F1 reveals a common regulation mechanism for androgen-responsive genes in prostate cells. / Altintas, D. M.; Shukla, M. S.; Goutte-Gattat, D.; Angelov, D.; Rouault, J. P.; Dimitrov, S.; Samarut, Jacques.

In: Molecular Endocrinology, Vol. 26, No. 9, 09.2012, p. 1531-1541.

Research output: Contribution to journalArticle

Altintas, D. M. ; Shukla, M. S. ; Goutte-Gattat, D. ; Angelov, D. ; Rouault, J. P. ; Dimitrov, S. ; Samarut, Jacques. / Direct cooperation between androgen receptor and E2F1 reveals a common regulation mechanism for androgen-responsive genes in prostate cells. In: Molecular Endocrinology. 2012 ; Vol. 26, No. 9. pp. 1531-1541.
@article{55c45ac84dad4fd1b328b82067ffb12d,
title = "Direct cooperation between androgen receptor and E2F1 reveals a common regulation mechanism for androgen-responsive genes in prostate cells",
abstract = "We have studied the regulation of ATAD2 gene expression by androgens in prostate cells. ATAD2 is a coactivator of the androgen receptor (AR) and the MYC protein. We showed that ATAD2 expression is directly regulated by AR via an AR binding sequence (ARBS) located in the distal enhancer of its regulatory region. The gene is also regulated by the E2F1 transcription factor. Using knockdown and chromatin immunoprecipitation technique approaches, we could demonstrate that AR and E2F1 functionally collaborate and physically interact between each other. From the analysis of chromatin conformation, we conclude that this cooperation results from a chromatin looping over the ATAD2 promoter region between the ARBS and E2F1 binding site in an androgen-dependent manner. Furthermore, we could show that several genes overexpressed in prostate cancer and potentially involved in several aspects of tumor development have an ARBS and an E2F1 binding site in their regulatory regions and exhibit the same mechanism of regulation by both transcription factors as ATAD2.",
author = "Altintas, {D. M.} and Shukla, {M. S.} and D. Goutte-Gattat and D. Angelov and Rouault, {J. P.} and S. Dimitrov and Jacques Samarut",
year = "2012",
month = "9",
doi = "10.1210/me.2012-1016",
language = "English",
volume = "26",
pages = "1531--1541",
journal = "Molecular Endocrinology",
issn = "0888-8809",
publisher = "The Endocrine Society",
number = "9",

}

TY - JOUR

T1 - Direct cooperation between androgen receptor and E2F1 reveals a common regulation mechanism for androgen-responsive genes in prostate cells

AU - Altintas, D. M.

AU - Shukla, M. S.

AU - Goutte-Gattat, D.

AU - Angelov, D.

AU - Rouault, J. P.

AU - Dimitrov, S.

AU - Samarut, Jacques

PY - 2012/9

Y1 - 2012/9

N2 - We have studied the regulation of ATAD2 gene expression by androgens in prostate cells. ATAD2 is a coactivator of the androgen receptor (AR) and the MYC protein. We showed that ATAD2 expression is directly regulated by AR via an AR binding sequence (ARBS) located in the distal enhancer of its regulatory region. The gene is also regulated by the E2F1 transcription factor. Using knockdown and chromatin immunoprecipitation technique approaches, we could demonstrate that AR and E2F1 functionally collaborate and physically interact between each other. From the analysis of chromatin conformation, we conclude that this cooperation results from a chromatin looping over the ATAD2 promoter region between the ARBS and E2F1 binding site in an androgen-dependent manner. Furthermore, we could show that several genes overexpressed in prostate cancer and potentially involved in several aspects of tumor development have an ARBS and an E2F1 binding site in their regulatory regions and exhibit the same mechanism of regulation by both transcription factors as ATAD2.

AB - We have studied the regulation of ATAD2 gene expression by androgens in prostate cells. ATAD2 is a coactivator of the androgen receptor (AR) and the MYC protein. We showed that ATAD2 expression is directly regulated by AR via an AR binding sequence (ARBS) located in the distal enhancer of its regulatory region. The gene is also regulated by the E2F1 transcription factor. Using knockdown and chromatin immunoprecipitation technique approaches, we could demonstrate that AR and E2F1 functionally collaborate and physically interact between each other. From the analysis of chromatin conformation, we conclude that this cooperation results from a chromatin looping over the ATAD2 promoter region between the ARBS and E2F1 binding site in an androgen-dependent manner. Furthermore, we could show that several genes overexpressed in prostate cancer and potentially involved in several aspects of tumor development have an ARBS and an E2F1 binding site in their regulatory regions and exhibit the same mechanism of regulation by both transcription factors as ATAD2.

UR - http://www.scopus.com/inward/record.url?scp=84865600685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865600685&partnerID=8YFLogxK

U2 - 10.1210/me.2012-1016

DO - 10.1210/me.2012-1016

M3 - Article

VL - 26

SP - 1531

EP - 1541

JO - Molecular Endocrinology

JF - Molecular Endocrinology

SN - 0888-8809

IS - 9

ER -