Differential responses of plasmacytoid dendritic cells to influenza virus and distinct viral pathogens

Jaime M. Thomas, Zoltan Pos, Jennifer Reinboth, Richard Y. Wang, Ena Wang, Gregory M. Frank, Paolo Lusso, Giorgio Trinchieri, Harvey J. Alter, Francesco M. Marincola, Emmanuel Thomas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Plasmacytoid dendritic cells (pDCs) are key components of the innate immune response that are capable of synthesizing and rapidly releasing vast amounts of type I interferons (IFNs), particularly IFN-α. Here we investigated whether pDCs, often regarded as a mere source of IFN, discriminate between various functionally discrete stimuli and to what extent this reflects differences in pDC responses other than IFN-α release. To examine the ability of pDCs to differentially respond to various doses of intact and infectious HIV, hepatitis C virus, and H1N1 influenza virus, whole-genome gene expression analysis, enzyme-linked immunosorbent assays, and flow cytometry were used to investigate pDC responses at the transcriptional, protein, and cellular levels. Our data demonstrate that pDCs respond differentially to various viral stimuli with significant changes in gene expression, including those involved in pDC activation, migration, viral endocytosis, survival, or apoptosis. In some cases, the expression of these genes was induced even at levels comparable to that of IFN-α. Interestingly, we also found that depending on the viral entity and the viral titer used for stimulation, induction of IFN-α gene expression and the actual release of IFN-α are not necessarily temporally coordinated. In addition, our data suggest that high-titer influenza A (H1N1) virus infection can stimulate rapid pDC apoptosis.

Original languageEnglish
Pages (from-to)10758-10766
Number of pages9
JournalJournal of Virology
Volume88
Issue number18
DOIs
Publication statusPublished - 2014

Fingerprint

Orthomyxoviridae
Dendritic Cells
Interferons
Gene Expression
H1N1 Subtype Influenza A Virus
Apoptosis
Virus Activation
Interferon Type I
Hepatitis A
Influenza A virus
Virus Diseases
Cellular Structures
Endocytosis
Innate Immunity
Hepacivirus
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
HIV
Genome
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Thomas, J. M., Pos, Z., Reinboth, J., Wang, R. Y., Wang, E., Frank, G. M., ... Thomas, E. (2014). Differential responses of plasmacytoid dendritic cells to influenza virus and distinct viral pathogens. Journal of Virology, 88(18), 10758-10766. https://doi.org/10.1128/JVI.01501-14

Differential responses of plasmacytoid dendritic cells to influenza virus and distinct viral pathogens. / Thomas, Jaime M.; Pos, Zoltan; Reinboth, Jennifer; Wang, Richard Y.; Wang, Ena; Frank, Gregory M.; Lusso, Paolo; Trinchieri, Giorgio; Alter, Harvey J.; Marincola, Francesco M.; Thomas, Emmanuel.

In: Journal of Virology, Vol. 88, No. 18, 2014, p. 10758-10766.

Research output: Contribution to journalArticle

Thomas, JM, Pos, Z, Reinboth, J, Wang, RY, Wang, E, Frank, GM, Lusso, P, Trinchieri, G, Alter, HJ, Marincola, FM & Thomas, E 2014, 'Differential responses of plasmacytoid dendritic cells to influenza virus and distinct viral pathogens', Journal of Virology, vol. 88, no. 18, pp. 10758-10766. https://doi.org/10.1128/JVI.01501-14
Thomas, Jaime M. ; Pos, Zoltan ; Reinboth, Jennifer ; Wang, Richard Y. ; Wang, Ena ; Frank, Gregory M. ; Lusso, Paolo ; Trinchieri, Giorgio ; Alter, Harvey J. ; Marincola, Francesco M. ; Thomas, Emmanuel. / Differential responses of plasmacytoid dendritic cells to influenza virus and distinct viral pathogens. In: Journal of Virology. 2014 ; Vol. 88, No. 18. pp. 10758-10766.
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