Differential remodeling of the HIV-1 nucleosome upon transcription activators and SWI/SNF complex binding

Dimitar Angelov, Monique Charra, Michel Seve, Jacques Côté, Saadi Khochbin, Stefan Dimitrov

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Here we have examined HIV-1 nucleosome remodeling upon the binding of transcription factors and the SWI/SNF complex using a novel approach. The approach combines UV laser protein-DNA crosslinking, electrophoretic mobility-shift analysis and DNase I protection analysis with immunochemical techniques. It was found that single activator-bound HIV-1 nucleosomes exhibit very weak perturbation in histone NH2 tail-DNA interactions. However, the simultaneous binding of the transcription activators Sp1, NF-kB1, LEF-1 and USF synergistically increased the release of histone NH2 tails from nucleosomal DNA. In contrast, the binding of SWI/SNF complex to HIV-1 nucleosome disrupted structured histone domain-DNA contacts, but not histone NH2 tail-DNA interactions. Stable remodeled nucleosomes, (obtained after detachment of SWI/SNF), displayed identical structural alterations with those bound to SWI/SNF. These results demonstrate a different in vitro remodeling of the HIV-1 nucleosome upon the binding of multiple transcription activators and of SWI/SNF complex. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)315-326
Number of pages12
JournalJournal of Molecular Biology
Volume302
Issue number2
DOIs
Publication statusPublished - 15 Sep 2000
Externally publishedYes

Fingerprint

Nucleosomes
HIV-1
Histones
DNA
Deoxyribonuclease I
Lasers
Transcription Factors
Proteins

Keywords

  • Activators
  • Histone tails
  • Nucleosome
  • Remodeling
  • SWI/SNF complex

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Differential remodeling of the HIV-1 nucleosome upon transcription activators and SWI/SNF complex binding. / Angelov, Dimitar; Charra, Monique; Seve, Michel; Côté, Jacques; Khochbin, Saadi; Dimitrov, Stefan.

In: Journal of Molecular Biology, Vol. 302, No. 2, 15.09.2000, p. 315-326.

Research output: Contribution to journalArticle

Angelov, Dimitar ; Charra, Monique ; Seve, Michel ; Côté, Jacques ; Khochbin, Saadi ; Dimitrov, Stefan. / Differential remodeling of the HIV-1 nucleosome upon transcription activators and SWI/SNF complex binding. In: Journal of Molecular Biology. 2000 ; Vol. 302, No. 2. pp. 315-326.
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