Differential regulation of transforming growth factor β and interleukin 2 genes in human T cells: Demonstration by usage of novel competitor DNA constructs in the quantitative polymerase chain reaction

B. Li, P. K. Sehajpal, A. Khanna, H. Vlassara, A. Cerami, K. H. Stenzel, Manikkam Suthanthiran

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

The regulation of mKNA encoding transforming growth factor β (TGF-β) and interleukin 2 (IL,2) in normal human T cells was explored using novel competitor DNA constructs in the quantitative polymerase chain reaction and accessory cell-independent T cell activation models. Our experimental design revealed the following: (a) TGF-β mRNA and IL-2 mRNA are regulated differentially in normal human T cells, quiescent or signaled with the synergistic combinations of: sn-l,2-dioctanoylglycerol and ionomycin or anti-CD3 monoclonal antibody (mAb) and anti- CD2 mAb; (b) the steady-state level of TGF-β mRNA in the stimulated T cells, in contrast to that of II.2 mRNA, is increased by the immunosuppressant cyclosporine (CsA); and (c) the paradoxical effect of CsA on TGF-β mRNA levels is also appreciable at the level of production of functionally active TGF-β protein. Our findings, in addition to demonstrating the utility of the competitor DNA constructs for the precise quantification of immunoregulatory cytokines, suggest a novel and unifying mechanistic basis for the immunosuppression and some of the complications (e.g., renal fibrosis) associated with CsA usage.

Original languageEnglish
Pages (from-to)1259-1262
Number of pages4
JournalJournal of Experimental Medicine
Volume174
Issue number5
DOIs
Publication statusPublished - 1 Nov 1991
Externally publishedYes

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Transforming Growth Factors
Interleukin-2
T-Lymphocytes
Polymerase Chain Reaction
Messenger RNA
DNA
Genes
Monoclonal Antibodies
Ionomycin
Immunosuppressive Agents
Immunosuppression
Cyclosporine
Fibrosis
Research Design
Cytokines
Kidney
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Differential regulation of transforming growth factor β and interleukin 2 genes in human T cells : Demonstration by usage of novel competitor DNA constructs in the quantitative polymerase chain reaction. / Li, B.; Sehajpal, P. K.; Khanna, A.; Vlassara, H.; Cerami, A.; Stenzel, K. H.; Suthanthiran, Manikkam.

In: Journal of Experimental Medicine, Vol. 174, No. 5, 01.11.1991, p. 1259-1262.

Research output: Contribution to journalArticle

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