Differential effects and potential adverse outcomes of ionic silver and silver nanoparticles in vivo and in vitro

Natália Garcia-Reyero, Alan J. Kennedy, B. Lynn Escalon, Tanwir Habib, Jennifer G. Laird, Arun Rawat, Steven Wiseman, Markus Hecker, Nancy Denslow, Jeffery A. Steevens, Edward J. Perkins

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Nanoparticles are of concern because of widespread use, but it is unclear if metal nanoparticles cause effects directly or indirectly. We explored whether polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) cause effects through intact nanoparticles or dissolved silver. Females of the model species fathead minnow (Pimephales promelas) were exposed to either 4.8 μg/L of AgNO3 or 61.4 μg/L of PVP-AgNPs for 96h. Microarray analyses were used to identify impacted receptors and toxicity pathways in liver and brain tissues that were confirmed using in vitro mammalian assays. AgNO3 and PVP-AgNP exposed fish had common and distinct effects consistent with both intact nanoparticles and dissolved silver causing effects. PVP-AgNPs and AgNO3 both affected pathways involved in Na+, K +, and H+ homeostasis and oxidative stress but different neurotoxicity pathways. In vivo effects were supported by PVP-AgNP activation of five in vitro nuclear receptor assays and inhibition of ligand binding to the dopamine receptor. AgNO3 inhibited ligand binding to adrenergic receptors α1 and α2 and cannabinoid receptor CB1, but had no effect in nuclear receptor assays. PVP-AgNPs have the potential to cause effects both through intact nanoparticles and metal ions, each interacting with different initiating events. Since the in vitro and in vivo assays examined here are commonly used in human and ecological hazard screening, this work suggests that environmental health assessments should consider effects of intact nanoparticles in addition to dissolved metals.

Original languageEnglish
Pages (from-to)4546-4555
Number of pages10
JournalEnvironmental Science and Technology
Volume48
Issue number8
DOIs
Publication statusPublished - 15 Apr 2014
Externally publishedYes

Fingerprint

Silver
silver
Nanoparticles
Assays
Cytoplasmic and Nuclear Receptors
ligand
Ligands
Cannabinoid Receptor CB1
Povidone
metal
Oxidative stress
Metal nanoparticles
Dopamine Receptors
assay
Microarrays
Liver
Fish
Adrenergic Receptors
Metal ions
Toxicity

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Differential effects and potential adverse outcomes of ionic silver and silver nanoparticles in vivo and in vitro. / Garcia-Reyero, Natália; Kennedy, Alan J.; Escalon, B. Lynn; Habib, Tanwir; Laird, Jennifer G.; Rawat, Arun; Wiseman, Steven; Hecker, Markus; Denslow, Nancy; Steevens, Jeffery A.; Perkins, Edward J.

In: Environmental Science and Technology, Vol. 48, No. 8, 15.04.2014, p. 4546-4555.

Research output: Contribution to journalArticle

Garcia-Reyero, N, Kennedy, AJ, Escalon, BL, Habib, T, Laird, JG, Rawat, A, Wiseman, S, Hecker, M, Denslow, N, Steevens, JA & Perkins, EJ 2014, 'Differential effects and potential adverse outcomes of ionic silver and silver nanoparticles in vivo and in vitro', Environmental Science and Technology, vol. 48, no. 8, pp. 4546-4555. https://doi.org/10.1021/es4042258
Garcia-Reyero, Natália ; Kennedy, Alan J. ; Escalon, B. Lynn ; Habib, Tanwir ; Laird, Jennifer G. ; Rawat, Arun ; Wiseman, Steven ; Hecker, Markus ; Denslow, Nancy ; Steevens, Jeffery A. ; Perkins, Edward J. / Differential effects and potential adverse outcomes of ionic silver and silver nanoparticles in vivo and in vitro. In: Environmental Science and Technology. 2014 ; Vol. 48, No. 8. pp. 4546-4555.
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