Different modalities of intercellular membrane exchanges mediate cell-to-cell P-glycoprotein transfers in MCF-7 breast cancer cells

Jennifer Pasquier, Ludovic Galas, Céline Boulangé-Lecomte, Damien Rioult, Florence Bultelle, Pierre Magal, Glenn Webb, Frank Le Foll

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Multi-drug resistance (MDR) is a phenomenon by which tumor cells exhibit resistance to a variety of chemically unrelated chemotherapeutic drugs. The classical form of multidrug resistance is connected to overexpression of membrane P-glycoprotein (P-gp), which acts as an energy dependent drug efflux pump. P-glycoprotein expression is known to be controlled by genetic and epigenetic mechanisms. Untilnowprocesses of P-gp gene up-regulation and resistant cell selection were considered sufficient to explain the emergence of MDR phenotype within a cell population. Recently, however, "non- genetic" acquisitions of MDR by cell-to-cell P-gp transfers have been pointed out. In the present study we show that intercellular transfers of functional P-gp occur by two different but complementary modalities through donor-recipient cells interactions in the absence of drug selection pressure. P-glycoprotein and drug efflux activity transfers were followed over 7 days by confocal microscopy and flow cytometry in drug-sensitive parental MCF-7 breast cancer cells co-cultured with P-gp overexpressing resistant variants. An early process of remote transfer was established based on the release and binding of P-gp-containing microparticles. Microparticle- mediated transfers were detected after only 4 h of incubation. We also identify an alternative mode of transfer by contact, consisting of cell-to-cell P-gp trafficking by tunneling nanotubes bridging neighboring cells. Our findings supply new mechanistic evidences for the extragenetic emergence of MDR in cancer cells and indicate that new treatment strategies designed to overcome MDR may include inhibition of both microparticles and Tunneling nanotube-mediated intercellular P-gp transfers.

Original languageEnglish
Pages (from-to)7374-7387
Number of pages14
JournalJournal of Biological Chemistry
Volume287
Issue number10
DOIs
Publication statusPublished - 2 Mar 2012
Externally publishedYes

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P-Glycoprotein
Cells
Breast Neoplasms
Membranes
Multiple Drug Resistance
Pharmaceutical Preparations
Nanotubes
Flow cytometry
Confocal microscopy
Epigenomics
Confocal Microscopy
Cell Communication
Tumors
Cultured Cells
Neoplasms
Flow Cytometry
Up-Regulation
Genes
Pumps
Phenotype

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Different modalities of intercellular membrane exchanges mediate cell-to-cell P-glycoprotein transfers in MCF-7 breast cancer cells. / Pasquier, Jennifer; Galas, Ludovic; Boulangé-Lecomte, Céline; Rioult, Damien; Bultelle, Florence; Magal, Pierre; Webb, Glenn; Le Foll, Frank.

In: Journal of Biological Chemistry, Vol. 287, No. 10, 02.03.2012, p. 7374-7387.

Research output: Contribution to journalArticle

Pasquier, Jennifer ; Galas, Ludovic ; Boulangé-Lecomte, Céline ; Rioult, Damien ; Bultelle, Florence ; Magal, Pierre ; Webb, Glenn ; Le Foll, Frank. / Different modalities of intercellular membrane exchanges mediate cell-to-cell P-glycoprotein transfers in MCF-7 breast cancer cells. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 10. pp. 7374-7387.
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