Differences between human plasma and serum metabolite profiles

Zhonghao Yu, Gabi Kastenmüller, Ying He, Petra Belcredi, Gabriele Möller, Cornelia Prehn, Joaquim Mendes, Simone Wahl, Werner Roemisch-Margl, Uta Ceglarek, Alexey Polonikov, Norbert Dahmen, Holger Prokisch, Lu Xie, Yixue Li, H. Erich Wichmann, Annette Peters, Florian Kronenberg, Karsten Suhre, Jerzy AdamskiThomas Illig, Rui Wang-Sattler

Research output: Contribution to journalArticle

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Abstract

Background: Human plasma and serum are widely used matrices in clinical and biological studies. However, different collecting procedures and the coagulation cascade influence concentrations of both proteins and metabolites in these matrices. The effects on metabolite concentration profiles have not been fully characterized. Methodology/Principal Findings: We analyzed the concentrations of 163 metabolites in plasma and serum samples collected simultaneously from 377 fasting individuals. To ensure data quality, 41 metabolites with low measurement stability were excluded from further analysis. In addition, plasma and corresponding serum samples from 83 individuals were re-measured in the same plates and mean correlation coefficients (r) of all metabolites between the duplicates were 0.83 and 0.80 in plasma and serum, respectively, indicating significantly better stability of plasma compared to serum (p = 0.01). Metabolite profiles from plasma and serum were clearly distinct with 104 metabolites showing significantly higher concentrations in serum. In particular, 9 metabolites showed relative concentration differences larger than 20%. Despite differences in absolute concentration between the two matrices, for most metabolites the overall correlation was high (mean r = 0.81±0.10), which reflects a proportional change in concentration. Furthermore, when two groups of individuals with different phenotypes were compared with each other using both matrices, more metabolites with significantly different concentrations could be identified in serum than in plasma. For example, when 51 type 2 diabetes (T2D) patients were compared with 326 non-T2D individuals, 15 more significantly different metabolites were found in serum, in addition to the 25 common to both matrices. Conclusions/Significance: Our study shows that reproducibility was good in both plasma and serum, and better in plasma. Furthermore, as long as the same blood preparation procedure is used, either matrix should generate similar results in clinical and biological studies. The higher metabolite concentrations in serum, however, make it possible to provide more sensitive results in biomarker detection.

Original languageEnglish
Article numbere21230
JournalPLoS One
Volume6
Issue number7
DOIs
Publication statusPublished - 2011
Externally publishedYes

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Plasma (human)
Metabolites
blood serum
metabolites
Serum
Plasmas
Medical problems
Biomarkers
Coagulation
Type 2 Diabetes Mellitus
coagulation
noninsulin-dependent diabetes mellitus
reproducibility
Fasting
fasting
diabetes
biomarkers

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Yu, Z., Kastenmüller, G., He, Y., Belcredi, P., Möller, G., Prehn, C., ... Wang-Sattler, R. (2011). Differences between human plasma and serum metabolite profiles. PLoS One, 6(7), [e21230]. https://doi.org/10.1371/journal.pone.0021230

Differences between human plasma and serum metabolite profiles. / Yu, Zhonghao; Kastenmüller, Gabi; He, Ying; Belcredi, Petra; Möller, Gabriele; Prehn, Cornelia; Mendes, Joaquim; Wahl, Simone; Roemisch-Margl, Werner; Ceglarek, Uta; Polonikov, Alexey; Dahmen, Norbert; Prokisch, Holger; Xie, Lu; Li, Yixue; Wichmann, H. Erich; Peters, Annette; Kronenberg, Florian; Suhre, Karsten; Adamski, Jerzy; Illig, Thomas; Wang-Sattler, Rui.

In: PLoS One, Vol. 6, No. 7, e21230, 2011.

Research output: Contribution to journalArticle

Yu, Z, Kastenmüller, G, He, Y, Belcredi, P, Möller, G, Prehn, C, Mendes, J, Wahl, S, Roemisch-Margl, W, Ceglarek, U, Polonikov, A, Dahmen, N, Prokisch, H, Xie, L, Li, Y, Wichmann, HE, Peters, A, Kronenberg, F, Suhre, K, Adamski, J, Illig, T & Wang-Sattler, R 2011, 'Differences between human plasma and serum metabolite profiles', PLoS One, vol. 6, no. 7, e21230. https://doi.org/10.1371/journal.pone.0021230
Yu Z, Kastenmüller G, He Y, Belcredi P, Möller G, Prehn C et al. Differences between human plasma and serum metabolite profiles. PLoS One. 2011;6(7). e21230. https://doi.org/10.1371/journal.pone.0021230
Yu, Zhonghao ; Kastenmüller, Gabi ; He, Ying ; Belcredi, Petra ; Möller, Gabriele ; Prehn, Cornelia ; Mendes, Joaquim ; Wahl, Simone ; Roemisch-Margl, Werner ; Ceglarek, Uta ; Polonikov, Alexey ; Dahmen, Norbert ; Prokisch, Holger ; Xie, Lu ; Li, Yixue ; Wichmann, H. Erich ; Peters, Annette ; Kronenberg, Florian ; Suhre, Karsten ; Adamski, Jerzy ; Illig, Thomas ; Wang-Sattler, Rui. / Differences between human plasma and serum metabolite profiles. In: PLoS One. 2011 ; Vol. 6, No. 7.
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AU - He, Ying

AU - Belcredi, Petra

AU - Möller, Gabriele

AU - Prehn, Cornelia

AU - Mendes, Joaquim

AU - Wahl, Simone

AU - Roemisch-Margl, Werner

AU - Ceglarek, Uta

AU - Polonikov, Alexey

AU - Dahmen, Norbert

AU - Prokisch, Holger

AU - Xie, Lu

AU - Li, Yixue

AU - Wichmann, H. Erich

AU - Peters, Annette

AU - Kronenberg, Florian

AU - Suhre, Karsten

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AU - Illig, Thomas

AU - Wang-Sattler, Rui

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