Development of Activity-Based Probes for Imaging Human α- L -Fucosidases in Cells

Yu Ling Hsu, Manjula Nandakumar, Hsin Yi Lai, Tzyy Chao Chou, Chi Yuan Chu, Chun Hung Lin, Lee Chiang Lo

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9 Citations (Scopus)

Abstract

We have established a concise synthetic route relying on a key base-promoted epimerization step to synthesize two series of activity-based probes carrying a BODIPY fluorophore for α-l-fucosidase. The resulting probes were evaluated for labeling performance. The one utilizing an o-fluoromethylphenol derivative as the latent trapping unit was successfully applied for the first time to visualize and locate lysosomal α-l-fucosidase activity in human cells.

Original languageEnglish
Pages (from-to)8458-8463
Number of pages6
JournalJournal of Organic Chemistry
Volume80
Issue number16
DOIs
Publication statusPublished - 21 Aug 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Organic Chemistry

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