Development of Activity-Based Probes for Imaging Human α- L -Fucosidases in Cells

Yu Ling Hsu, Manjula Nandakumar, Hsin Yi Lai, Tzyy Chao Chou, Chi Yuan Chu, Chun Hung Lin, Lee Chiang Lo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have established a concise synthetic route relying on a key base-promoted epimerization step to synthesize two series of activity-based probes carrying a BODIPY fluorophore for α-l-fucosidase. The resulting probes were evaluated for labeling performance. The one utilizing an o-fluoromethylphenol derivative as the latent trapping unit was successfully applied for the first time to visualize and locate lysosomal α-l-fucosidase activity in human cells.

Original languageEnglish
Pages (from-to)8458-8463
Number of pages6
JournalJournal of Organic Chemistry
Volume80
Issue number16
DOIs
Publication statusPublished - 21 Aug 2015
Externally publishedYes

Fingerprint

alpha-L-Fucosidase
Imaging techniques
Fluorophores
Human Activities
Labeling
Cells
Derivatives
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Development of Activity-Based Probes for Imaging Human α- L -Fucosidases in Cells. / Hsu, Yu Ling; Nandakumar, Manjula; Lai, Hsin Yi; Chou, Tzyy Chao; Chu, Chi Yuan; Lin, Chun Hung; Lo, Lee Chiang.

In: Journal of Organic Chemistry, Vol. 80, No. 16, 21.08.2015, p. 8458-8463.

Research output: Contribution to journalArticle

Hsu, Yu Ling ; Nandakumar, Manjula ; Lai, Hsin Yi ; Chou, Tzyy Chao ; Chu, Chi Yuan ; Lin, Chun Hung ; Lo, Lee Chiang. / Development of Activity-Based Probes for Imaging Human α- L -Fucosidases in Cells. In: Journal of Organic Chemistry. 2015 ; Vol. 80, No. 16. pp. 8458-8463.
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