Development and characterization of melanoma cell lines established by fine-needle aspiration biopsy

Advances in the monitoring of patients with metastatic melanoma

A. I. Riker, M. C. Panelli, U. S. Kammula, E. Wang, J. Wunderlich, A. Abati, P. Fetsch, S. A. Rosenberg, F. M. Marincola

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The establishment of melanoma cell lines from fine-needle aspiration biopsies (FNAB) has allowed for an enhanced understanding of the complex interactions that occur between T cells and tumor cells. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment. The expression of melanoma antigens (MAs) was assessed in fresh melanoma FNAB samples and from tumor cell lines derived from these samples using several different approaches. Cytospin preparations of freshly isolated tumor cell explants were analyzed by immunocytochemistry (ICC), while the daughter cell line was analyzed by fluorescent activated cell sorting (FACS) analysis, and semiquantitative and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR, qRT-PCR). As assessed by these methods, the level of MA expression by the original tumor cell explants correlated with the expression in established in vitro cell lines. Molecular analysis of the established cell lines utilizing PCR technology improved the sensitivity of detection of MA expression. Thus FNAB of melanoma is an efficient and effective method of tissue procurement, capable of generating, sequentially and from the same lesion, fresh tumor cells, tumor infiltrating lymphocytes (TIL), and long-term melanoma cell lines.

Original languageEnglish
Pages (from-to)387-396
Number of pages10
JournalCancer Detection and Prevention
Volume23
Issue number5
DOIs
Publication statusPublished - 1999
Externally publishedYes

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Physiologic Monitoring
Fine Needle Biopsy
Melanoma
Melanoma-Specific Antigens
Cell Line
Neoplasms
Polymerase Chain Reaction
Tumor-Infiltrating Lymphocytes
Tissue and Organ Procurement
Tumor Cell Line
Reverse Transcriptase Polymerase Chain Reaction
Immunohistochemistry
Technology
T-Lymphocytes

Keywords

  • Fine-needle aspiration biopsy
  • Melanoma
  • Tumor antigens

ASJC Scopus subject areas

  • Oncology

Cite this

Development and characterization of melanoma cell lines established by fine-needle aspiration biopsy : Advances in the monitoring of patients with metastatic melanoma. / Riker, A. I.; Panelli, M. C.; Kammula, U. S.; Wang, E.; Wunderlich, J.; Abati, A.; Fetsch, P.; Rosenberg, S. A.; Marincola, F. M.

In: Cancer Detection and Prevention, Vol. 23, No. 5, 1999, p. 387-396.

Research output: Contribution to journalArticle

Riker, A. I. ; Panelli, M. C. ; Kammula, U. S. ; Wang, E. ; Wunderlich, J. ; Abati, A. ; Fetsch, P. ; Rosenberg, S. A. ; Marincola, F. M. / Development and characterization of melanoma cell lines established by fine-needle aspiration biopsy : Advances in the monitoring of patients with metastatic melanoma. In: Cancer Detection and Prevention. 1999 ; Vol. 23, No. 5. pp. 387-396.
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