Determination of apoptosis sensitivity in specific t cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique

Bernice Lo, Madhu Ramaswamy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Among the different techniques available for determining physiological cell death or apoptosis in immune cells, fl uorescence-activated cell sorting-based approaches prove to be one of the most ef fi cient and quantitative assays in capturing cells that are actively undergoing apoptosis elicited by either extrinsic or intrinsic forms of cell death. The key advantage of the technique is to allow the user to determine apoptotic responses of multiple immune cell types or subsets of the same lineage of immune cells without the prior requirement for cell separation. Here, we describe a "multiparameter" fl ow method to rapidly determine apoptosis-sensitivity induced by the Fas receptor pathway within different CD4 T cell subsets in a mixed pool of analyzed ex vivo peripheral blood mononuclear cells.

Original languageEnglish
Title of host publicationImmune Homeostasis: Methods and Protocols
PublisherHumana Press Inc.
Pages33-41
Number of pages9
Volume979
ISBN (Print)9781627032896
DOIs
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume979
ISSN (Print)10643745

Fingerprint

Flow Cytometry
Apoptosis
Cell Death
CD95 Antigens
Cell Separation
T-Lymphocyte Subsets
Blood Cells

Keywords

  • Annexin
  • Apoptosis
  • Autoimmune Lymphoproliferative Syndrome (ALPS)
  • Autoimmunity
  • Ex vivo
  • Fas
  • Flow cytometry
  • Multi-parameter
  • T cell subsets

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Lo, B., & Ramaswamy, M. (2013). Determination of apoptosis sensitivity in specific t cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique. In Immune Homeostasis: Methods and Protocols (Vol. 979, pp. 33-41). (Methods in Molecular Biology; Vol. 979). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-290-2_4

Determination of apoptosis sensitivity in specific t cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique. / Lo, Bernice; Ramaswamy, Madhu.

Immune Homeostasis: Methods and Protocols. Vol. 979 Humana Press Inc., 2013. p. 33-41 (Methods in Molecular Biology; Vol. 979).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lo, B & Ramaswamy, M 2013, Determination of apoptosis sensitivity in specific t cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique. in Immune Homeostasis: Methods and Protocols. vol. 979, Methods in Molecular Biology, vol. 979, Humana Press Inc., pp. 33-41. https://doi.org/10.1007/978-1-62703-290-2_4
Lo, Bernice ; Ramaswamy, Madhu. / Determination of apoptosis sensitivity in specific t cell subsets from human peripheral blood by utilizing a multiparameter fluorescence-activated cell sorting-based technique. Immune Homeostasis: Methods and Protocols. Vol. 979 Humana Press Inc., 2013. pp. 33-41 (Methods in Molecular Biology).
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