Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry

The involvement of several pathways in tumorigenesis

Karim Chahed, Maria Kabbage, Bechr Hamrita, Christelle Lemaitre Guillier, Mounir Trimeche, Sami Remadi, Laurence Ehret-Sabatier, Lotfi Chouchane

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Little emphasis has been placed today on the elucidation of protein alterations in male breast carcinogenesis. Methods: Protein extracts were subjected to both isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoretic (NEPHGE) analyses. Differentially expressed proteins in tumor tissues were identified by matrix assisted laser desorption /ionization time of flight (MALDI-TOF) mass spectrometry and database search. Results: Some of the alterations involve variations in the expression of cytokeratins 8, 18 and 19. More interestingly, tropomyosin1, a protein known to play a role in suppression of the malignant phenotype, was found to be under-expressed in cancer tissues, implicating a possible pivotal role for this protein in male breast carcinogenesis. Co-upregulation of molecular chaperones (heat shock protein HSP27 and protein disulfide isomerase), stress related proteins (peroxiredoxin 1 and peptidylprolyl isomerase A) and glycolytic enzymes (enolase 1) occurred also in male breast tumors. Some of the remaining alterations include proteins involved in invasion and metastasis, such as galectin 1 and cathepsin D. Conclusions: The present study represents a first proteomic investigation of protein alterations in infiltrating ductal carcinomas (IDCA) of the male breast. A number of protein alterations in tumor tissues have been characterised thus, providing new insights into the molecular mechanisms underlying this disease.

Original languageEnglish
Pages (from-to)106-114
Number of pages9
JournalClinica Chimica Acta
Volume388
Issue number1-2
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

Fingerprint

Male Breast Neoplasms
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Mass spectrometry
Mass Spectrometry
Carcinogenesis
Gels
Proteins
Tumors
Heat-Shock Proteins
Tissue
Breast
Galectin 1
HSP27 Heat-Shock Proteins
Keratin-8
Peptidylprolyl Isomerase
Keratin-18
Protein Disulfide-Isomerases
Keratin-19
Carcinoma, Ductal, Breast

Keywords

  • Infiltrating ductal carcinomas
  • Male breast cancer
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry : The involvement of several pathways in tumorigenesis. / Chahed, Karim; Kabbage, Maria; Hamrita, Bechr; Guillier, Christelle Lemaitre; Trimeche, Mounir; Remadi, Sami; Ehret-Sabatier, Laurence; Chouchane, Lotfi.

In: Clinica Chimica Acta, Vol. 388, No. 1-2, 02.2008, p. 106-114.

Research output: Contribution to journalArticle

Chahed, Karim ; Kabbage, Maria ; Hamrita, Bechr ; Guillier, Christelle Lemaitre ; Trimeche, Mounir ; Remadi, Sami ; Ehret-Sabatier, Laurence ; Chouchane, Lotfi. / Detection of protein alterations in male breast cancer using two dimensional gel electrophoresis and mass spectrometry : The involvement of several pathways in tumorigenesis. In: Clinica Chimica Acta. 2008 ; Vol. 388, No. 1-2. pp. 106-114.
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