Detection of memory cells by chemical modification of the lymphocyte cell surface.

K. H. Stenzel, Manikkam Suthanthiran, A. L. Rubin, A. Novogrodsky

Research output: Contribution to journalArticle

Abstract

Stringent alloantigen requirements, necessary for the differentiation of human memory cells into specific secondary cytolytic T cells (2 degrees CTL), can be bypassed by chemical modification of memory cells with the mitogenic oxidising agent, galactose oxidase. Treatment of memory cells generated in a long-term primary mixed lymphocyte culture with neuraminidase and galactose oxidase (NAGO) results in the differentiation of memory cells into 2 degrees CTL. In contrast, treatment of unprimed cells with NAGO does not result in CTL production despite the proliferation resulting from such treatment.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalProceedings of the European Dialysis and Transplant Association. European Dialysis and Transplant Association
Volume16
Publication statusPublished - 1 Dec 1979
Externally publishedYes

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Galactose Oxidase
Lymphocytes
Neuraminidase
Isoantigens
Oxidants
Cell Differentiation
T-Lymphocytes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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