Detection of Human α- L -Fucosidases by a Quinone Methide-Generating Probe

Enhanced Activities in Response to Helicobacter pylori Infection

Manjula Nandakumar, Yu Ling Hsu, Jason C Y Lin, Chun Lo, Lee Chiang Lo, Chun Hung Lin

Research output: Contribution to journalArticle

Abstract

α-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human α-L-fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor. Probing for answers: We monitored α-L-fucosidase activity in vitro and in vivo using a quinone methide-based probe. The probe was applied for detecting increases in the activity of human fucosidases 1 and 2 in gastric epithelial cells upon H. pylori infection, with lipopolysaccharides identified as a primary contributing factor.

Original languageEnglish
Pages (from-to)1555-1559
Number of pages5
JournalChemBioChem
Volume16
Issue number11
DOIs
Publication statusPublished - 1 Jul 2015
Externally publishedYes

Fingerprint

alpha-L-Fucosidase
Helicobacter Infections
Helicobacter pylori
Human Activities
Lipopolysaccharides
Stomach
Epithelial Cells
Bacterial Infections
quinone methide

Keywords

  • activity-based probes
  • fucosidase
  • Helicobacter pylori
  • lipopolysaccharides
  • quinone methide

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Cite this

Detection of Human α- L -Fucosidases by a Quinone Methide-Generating Probe : Enhanced Activities in Response to Helicobacter pylori Infection. / Nandakumar, Manjula; Hsu, Yu Ling; Lin, Jason C Y; Lo, Chun; Lo, Lee Chiang; Lin, Chun Hung.

In: ChemBioChem, Vol. 16, No. 11, 01.07.2015, p. 1555-1559.

Research output: Contribution to journalArticle

Nandakumar, Manjula ; Hsu, Yu Ling ; Lin, Jason C Y ; Lo, Chun ; Lo, Lee Chiang ; Lin, Chun Hung. / Detection of Human α- L -Fucosidases by a Quinone Methide-Generating Probe : Enhanced Activities in Response to Helicobacter pylori Infection. In: ChemBioChem. 2015 ; Vol. 16, No. 11. pp. 1555-1559.
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