Depletion of trypanosome CTR9 leads to gene expression defects

Benard A. Ouna, Benson Nyambega, Theresa Manful, Claudia Helbig, Matilda Males, Abeer A. Fadda, Christine Clayton

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Paf complex of Opisthokonts and plants contains at least five subunits: Paf1, Cdc73, Rtf1, Ctr9, and Leo1. Mutations in, or loss of Paf complex subunits have been shown to cause defects in histone modification, mRNA polyadenylation, and transcription by RNA polymerase I and RNA polymerase II. We here investigated trypanosome CTR9, which is essential for trypanosome survival. The results of tandem affinity purification suggested that trypanosome CTR9 associates with homologues of Leo1 and Cdc73; genes encoding homologues of Rtf1 and Paf1 were not found. RNAi targeting CTR9 resulted in at least ten-fold decreases in 131 essential mRNAs: they included several that are required for gene expression and its control, such as those encoding subunits of RNA polymerases, exoribonucleases that target mRNA, RNA helicases and RNA-binding proteins. Simultaneously, some genes from regions subject to chromatin silencing were derepressed, possibly as a secondary effect of the loss of two proteins that are required for silencing, ISWI and NLP1.

Original languageEnglish
Article numbere34256
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - 20 Apr 2012
Externally publishedYes

Fingerprint

Trypanosomiasis
DNA-directed RNA polymerase
Gene expression
Gene Expression
gene expression
Messenger RNA
Defects
exoribonucleases
Histone Code
Exoribonucleases
RNA helicases
RNA Helicases
RNA Polymerase I
Polyadenylation
RNA-binding proteins
Gene encoding
RNA-Binding Proteins
RNA Polymerase II
DNA-Directed RNA Polymerases
Transcription

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ouna, B. A., Nyambega, B., Manful, T., Helbig, C., Males, M., Fadda, A. A., & Clayton, C. (2012). Depletion of trypanosome CTR9 leads to gene expression defects. PLoS One, 7(4), [e34256]. https://doi.org/10.1371/journal.pone.0034256

Depletion of trypanosome CTR9 leads to gene expression defects. / Ouna, Benard A.; Nyambega, Benson; Manful, Theresa; Helbig, Claudia; Males, Matilda; Fadda, Abeer A.; Clayton, Christine.

In: PLoS One, Vol. 7, No. 4, e34256, 20.04.2012.

Research output: Contribution to journalArticle

Ouna, BA, Nyambega, B, Manful, T, Helbig, C, Males, M, Fadda, AA & Clayton, C 2012, 'Depletion of trypanosome CTR9 leads to gene expression defects', PLoS One, vol. 7, no. 4, e34256. https://doi.org/10.1371/journal.pone.0034256
Ouna BA, Nyambega B, Manful T, Helbig C, Males M, Fadda AA et al. Depletion of trypanosome CTR9 leads to gene expression defects. PLoS One. 2012 Apr 20;7(4). e34256. https://doi.org/10.1371/journal.pone.0034256
Ouna, Benard A. ; Nyambega, Benson ; Manful, Theresa ; Helbig, Claudia ; Males, Matilda ; Fadda, Abeer A. ; Clayton, Christine. / Depletion of trypanosome CTR9 leads to gene expression defects. In: PLoS One. 2012 ; Vol. 7, No. 4.
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