Depletion of CD4 T lymphocytes in human lymphoid tissue infected ex vivo with doxycycline-dependent HIV-1

Yana Kiselyeva, Yoshinori Ito, Rosangela G. Lima, Jean-Charles B. Grivel, Atze T. Das, Ben Berkhout, Leonid B. Margolis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We investigated whether CD4 + T cells that do not produce HIV-1 are killed in HIV-infected human lymphoid tissue. Tissue blocks were inoculated with high amount of doxycycline-dependent HIV-rtTA. Doxycycline triggered productive infection and loss of CD4 + T cells in these tissues, whereas without doxycycline, neither productive infection nor CD4 + T cell depletion was detected in spite of the massive presence of virions in the tissue and of viral DNA in the cells. Thus, HIV-1 alone is sufficient to deplete productively infected CD4 + T cells but is not sufficient to cause the death of uninfected or latently infected CD4 + T cells.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalVirology
Volume328
Issue number1
DOIs
Publication statusPublished - 15 Oct 2004
Externally publishedYes

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Doxycycline
Lymphoid Tissue
HIV-1
T-Lymphocytes
HIV
Viral DNA
Infection
Virion
Cause of Death

Keywords

  • CD4 T cell
  • HIV-1
  • Human lymphoid tissue

ASJC Scopus subject areas

  • Virology

Cite this

Depletion of CD4 T lymphocytes in human lymphoid tissue infected ex vivo with doxycycline-dependent HIV-1. / Kiselyeva, Yana; Ito, Yoshinori; Lima, Rosangela G.; Grivel, Jean-Charles B.; Das, Atze T.; Berkhout, Ben; Margolis, Leonid B.

In: Virology, Vol. 328, No. 1, 15.10.2004, p. 1-6.

Research output: Contribution to journalArticle

Kiselyeva, Yana ; Ito, Yoshinori ; Lima, Rosangela G. ; Grivel, Jean-Charles B. ; Das, Atze T. ; Berkhout, Ben ; Margolis, Leonid B. / Depletion of CD4 T lymphocytes in human lymphoid tissue infected ex vivo with doxycycline-dependent HIV-1. In: Virology. 2004 ; Vol. 328, No. 1. pp. 1-6.
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