Depletion of CD4 T lymphocytes in human lymphoid tissue infected ex vivo with doxycycline-dependent HIV-1

Yana Kiselyeva, Yoshinori Ito, Rosangela G. Lima, Jean-Charles B. Grivel, Atze T. Das, Ben Berkhout, Leonid B. Margolis

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12 Citations (Scopus)

Abstract

We investigated whether CD4 + T cells that do not produce HIV-1 are killed in HIV-infected human lymphoid tissue. Tissue blocks were inoculated with high amount of doxycycline-dependent HIV-rtTA. Doxycycline triggered productive infection and loss of CD4 + T cells in these tissues, whereas without doxycycline, neither productive infection nor CD4 + T cell depletion was detected in spite of the massive presence of virions in the tissue and of viral DNA in the cells. Thus, HIV-1 alone is sufficient to deplete productively infected CD4 + T cells but is not sufficient to cause the death of uninfected or latently infected CD4 + T cells.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalVirology
Volume328
Issue number1
DOIs
Publication statusPublished - 15 Oct 2004
Externally publishedYes

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Keywords

  • CD4 T cell
  • HIV-1
  • Human lymphoid tissue

ASJC Scopus subject areas

  • Virology

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