Deoxygenation-linked association of a tetrameric component of chicken hemoglobin

John A. Cobb, David Manning, Prasanna Kolatkar, David J. Cox, Austen F. Riggs

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Deoxygenation-dependent association of hemoglobin tetramers appears to be widespread among amphibians, reptiles, and possibly all or most birds. The evidence for this conclusion depends largely on oxygen equilibria of whole blood which have Hill coefficients that reach values as high as 5-7 at 80-90% oxygenation. Computer simulation of the sedimentation velocity behavior of the major components A and D of chicken hemoglobin shows that component D but not A self-associates to form dimers of tetramers. The gradient profiles at pH 7.5 were satisfactorily fitted with an association constant of 1.26 × 104M-1 and sedimentation coefficients of 4.63 and 7.35 S for tetramer and (tetramer)2, respectively. Since components A and D share common β chains we conclude that tetramer-tetramer contacts must depend on surface residues of the α chains. Comparison of the amino acid sequences of the αD and αA chains of the hemoglobins from 12 avian species ranging from sparrow to ostrich shows that 20 residues are conserved in the αD chains but not in the αA chains. Nine of these (45%) are clustered between positions E20 and FG2. Four of the latter, Lys 71(E20), Asn75(EF4), Gln78(EF7), and Glu82(F3) are conserved in all αD chains even though they do not appear to participate in intratetramer contacts. Molecular modeling indicates that residues Lys71, Gln78, and Glu82 of the α chain are strong candidates for the primary tetramer-tetramer contacts.

Original languageEnglish
Pages (from-to)1183-1189
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number2
Publication statusPublished - 15 Jan 1992
Externally publishedYes

Fingerprint

Chickens
Hemoglobins
Struthioniformes
Sparrows
Sedimentation
Reptiles
Amphibians
Computer Simulation
Birds
Amino Acid Sequence
Molecular modeling
Oxygenation
Oxygen
Dimers
Blood
Amino Acids
Computer simulation
hemoglobin D
beryllium trifluoride

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cobb, J. A., Manning, D., Kolatkar, P., Cox, D. J., & Riggs, A. F. (1992). Deoxygenation-linked association of a tetrameric component of chicken hemoglobin. Journal of Biological Chemistry, 267(2), 1183-1189.

Deoxygenation-linked association of a tetrameric component of chicken hemoglobin. / Cobb, John A.; Manning, David; Kolatkar, Prasanna; Cox, David J.; Riggs, Austen F.

In: Journal of Biological Chemistry, Vol. 267, No. 2, 15.01.1992, p. 1183-1189.

Research output: Contribution to journalArticle

Cobb, JA, Manning, D, Kolatkar, P, Cox, DJ & Riggs, AF 1992, 'Deoxygenation-linked association of a tetrameric component of chicken hemoglobin', Journal of Biological Chemistry, vol. 267, no. 2, pp. 1183-1189.
Cobb, John A. ; Manning, David ; Kolatkar, Prasanna ; Cox, David J. ; Riggs, Austen F. / Deoxygenation-linked association of a tetrameric component of chicken hemoglobin. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 2. pp. 1183-1189.
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