Demonstration by in situ hybridization of dissimilar IL-1β gene expression in human alveolar macrophages and blood monocytes in response to lipopolysaccharide

J. F. Bernaudin, K. Yamauchi, M. D. Wewers, M. J. Tocci, V. J. Ferrans, Ronald Crystal

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Abstract

IL-1β is the major form of IL-1 produced by mononuclear phagocytes. To evaluate the possible mechanisms underlying the observation that mature populations of human mononuclear phagocytes as alveolar macrophages are relatively poor IL-1 producers compared with blood monocytes, the expression of the IL-1β gene mRNA transcripts was evaluated in LPS-stimulated autologous blood monocytes and alveolar macrophages by using a IL-1β cDNA probe. Although Northern analysis demonstrated that stimulated monocytes and alveolar macrophages both express 1.8-kb IL-1β mRNA transcripts, cytoplasmic dot blot analysis showed that the total IL-1β mRNA content in alveolar macrophages was only 38 ± 5% of that in blood monocytes. In situ hybridization with antisense and sense IL-1β RNA probes demonstrated that whereas most of stimulated blood monocytes contained IL-1β mRNA transcripts, a significant proportion of autologous alveolar macrophages stimulated in an identical fashion did not express the IL-1β gene. Within 4 h after LPS stimulation, IL-1β mRNA transcripts were detected in 81 ± 6% monocytes, whereas only 16 ± 9% of alveolar macrophages were positive, and by 18 h this had increased only to 43 ± 15%. Quantification of the size distribution of the IL-1β mRNA expressing mononuclear phagocytes demonstrated that, among the population of alveolar macrophages, the cells expressing this gene were not confined to those that were 'monocyte-like'. These observations demonstrate that there is a heterogeneity among population of mononuclear phagocytes in their ability to express the gene for IL-1β, which could explain the differences observed in IL-1 production.

Original languageEnglish
Pages (from-to)3822-3829
Number of pages8
JournalJournal of Immunology
Volume140
Issue number11
Publication statusPublished - 1 Jan 1988
Externally publishedYes

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Alveolar Macrophages
Interleukin-1
In Situ Hybridization
Lipopolysaccharides
Monocytes
Gene Expression
Phagocytes
Messenger RNA
Genes
RNA Probes
Alveolar Epithelial Cells
Population Characteristics
Population
Complementary DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Demonstration by in situ hybridization of dissimilar IL-1β gene expression in human alveolar macrophages and blood monocytes in response to lipopolysaccharide. / Bernaudin, J. F.; Yamauchi, K.; Wewers, M. D.; Tocci, M. J.; Ferrans, V. J.; Crystal, Ronald.

In: Journal of Immunology, Vol. 140, No. 11, 01.01.1988, p. 3822-3829.

Research output: Contribution to journalArticle

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abstract = "IL-1β is the major form of IL-1 produced by mononuclear phagocytes. To evaluate the possible mechanisms underlying the observation that mature populations of human mononuclear phagocytes as alveolar macrophages are relatively poor IL-1 producers compared with blood monocytes, the expression of the IL-1β gene mRNA transcripts was evaluated in LPS-stimulated autologous blood monocytes and alveolar macrophages by using a IL-1β cDNA probe. Although Northern analysis demonstrated that stimulated monocytes and alveolar macrophages both express 1.8-kb IL-1β mRNA transcripts, cytoplasmic dot blot analysis showed that the total IL-1β mRNA content in alveolar macrophages was only 38 ± 5{\%} of that in blood monocytes. In situ hybridization with antisense and sense IL-1β RNA probes demonstrated that whereas most of stimulated blood monocytes contained IL-1β mRNA transcripts, a significant proportion of autologous alveolar macrophages stimulated in an identical fashion did not express the IL-1β gene. Within 4 h after LPS stimulation, IL-1β mRNA transcripts were detected in 81 ± 6{\%} monocytes, whereas only 16 ± 9{\%} of alveolar macrophages were positive, and by 18 h this had increased only to 43 ± 15{\%}. Quantification of the size distribution of the IL-1β mRNA expressing mononuclear phagocytes demonstrated that, among the population of alveolar macrophages, the cells expressing this gene were not confined to those that were 'monocyte-like'. These observations demonstrate that there is a heterogeneity among population of mononuclear phagocytes in their ability to express the gene for IL-1β, which could explain the differences observed in IL-1 production.",
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