Delineation of the Salmonella enterica serovar Typhimurium HilA regulon through genome-wide location and transcript analysis

Inge M V Thijs, Sigrid C J De Keersmaecker, Abeer A. Fadda, Kristof Engelen, Hui Zhao, Michael McClelland, Kathleen Marchal, Jos Vanderleyden

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Abstract

The Salmonella enterica serovar Typhimurium HilA protein is the key regulator for the invasion of epithelial cells. By a combination of genome-wide location and transcript analysis, the HilA-dependent regulon has been delineated. Under invasion-inducing conditions, HilA binds to most of the known target genes and a number of new target genes. The sopB, sopE, and sopA genes, encoding effector proteins secreted by the type III secretion system on Salmonella pathogenicity island 1 (SPI-1), were identified as being both bound by HilA and differentially regulated in an HilA mutant. This suggests a cooperative role for HilA and InvF in the regulation of SPI-1-secreted effectors. Also, siiA, the first gene of SPI-4, is both bound by HilA and differentially regulated in an HilA mutant, thus linking this pathogenicity island to the invasion key regulator. Finally, the interactions of HilA with the SPI-2 secretion system gene ssaH and the flagellar gene flhD imply a repressor function for HilA under invasion-inducing conditions.

Original languageEnglish
Pages (from-to)4587-4596
Number of pages10
JournalJournal of Bacteriology
Volume189
Issue number13
DOIs
Publication statusPublished - Jul 2007
Externally publishedYes

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Thijs, I. M. V., De Keersmaecker, S. C. J., Fadda, A. A., Engelen, K., Zhao, H., McClelland, M., Marchal, K., & Vanderleyden, J. (2007). Delineation of the Salmonella enterica serovar Typhimurium HilA regulon through genome-wide location and transcript analysis. Journal of Bacteriology, 189(13), 4587-4596. https://doi.org/10.1128/JB.00178-07