In this paper, the design problem of delayed output feedback control scheme using two-layer interval fuzzy observers for a class of nonlinear systems with state and output delays is investigated. The Takagi-Sugeno type fuzzy linear model with an on-line update law is used to approximate the nonlinear system. Based on the fuzzy model, a two-layer interval fuzzy observer is used to reconstruct the system states according to equal interval output time delay slices. Subsequently, a delayed output feedback adaptive fuzzy controller is developed to override the nonlinearities, time delays, and external disturbances such that the H∞tracking performance is achieved. The linguistic information is developped by setting the membership functions of the fuzzy logic system and the adaptation parameters to estimate the model uncertainties directly for using linear analytical results instead of estimating nonlinear systemfunctions. The filtered tracking error dynamics are designed to satisfy the Strictly Positive Realness (SPR) condition. Based on the Lyapunov stability criterion and linear matrix inequalities (LMIs), some sufficient conditions are derived so that all states of the system are uniformly ultimately bounded and the effect of the external disturbances on the tracking error can be attenuated to any prescribed level and consequently an H ∞tracking control is achieved. Finally, a numerical example of a two-link robot manipulator is given to illustrate the effectiveness of the proposed control scheme.