Decreased expression of intelectin 1 in the human airway epithelium of smokers compared to nonsmokers

Brendan J. Carolan, Ben Gary Harvey, Bishnu P. De, Holly Vanni, Ronald Crystal

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Lectins are innate immune defense proteins that recognize bacterial cell wall components. Based on the knowledge that cigarette smoking is associated with an increased risk of infections, we hypothesized that cigarette smoking may modulate the expression of lectin genes in airway epithelium. Affymetrix microarrays were used to survey the expression of lectin genes in large airway epithelium from nine nonsmokers and 20 healthy smokers and in small airway epithelium from 13 nonsmokers and 20 healthy smokers. There were no changes (>2-fold change; p < 0.05) in lectin gene expression among healthy smokers compared with nonsmokers except for down-regulation of intelectin 1, a lectin that binds to galactofuranosyl residues in bacterial cell walls (large airway epithelium, p < 0.01; small airway epithelium, p < 0.01). This was confirmed by TaqMan RT-PCR in both large (p < 0.05) and small airway epithelium (p < 0.02). Immunohistochemistry assessment of airway biopsies demonstrated that intelectin 1 was expressed in secretory cells, while Western analysis confirmed the decreased expression of intelectin 1 in airway epithelium of healthy smokers compared with healthy nonsmokers (p < 0.02). Finally, compared with healthy nonsmokers, intelectin 1 expression was also decreased in small airway epithelium of smokers with lone emphysema and normal spirometry (n = 13, p < 0.01) and smokers with established chronic obstructive pulmonary disease (n = 14, p < 0.01). In the context that intelectin 1 plays a role in defense against bacteria, its down-regulation in response to cigarette smoking is another example of the immunomodulatory effects of smoking on the immune system and may contribute to the increase in susceptibility to infections observed in smokers.

Original languageEnglish
Pages (from-to)5760-5767
Number of pages8
JournalJournal of Immunology
Volume181
Issue number8
DOIs
Publication statusPublished - 1 Jan 2008
Externally publishedYes

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Epithelium
Lectins
Smoking
Gene Expression
Cell Wall
Down-Regulation
Bacterial Proteins
human ITLN1 protein
Spirometry
Emphysema
Cellular Structures
Infection
Chronic Obstructive Pulmonary Disease
Immune System
Immunohistochemistry
Bacteria
Biopsy
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Decreased expression of intelectin 1 in the human airway epithelium of smokers compared to nonsmokers. / Carolan, Brendan J.; Harvey, Ben Gary; De, Bishnu P.; Vanni, Holly; Crystal, Ronald.

In: Journal of Immunology, Vol. 181, No. 8, 01.01.2008, p. 5760-5767.

Research output: Contribution to journalArticle

Carolan, Brendan J. ; Harvey, Ben Gary ; De, Bishnu P. ; Vanni, Holly ; Crystal, Ronald. / Decreased expression of intelectin 1 in the human airway epithelium of smokers compared to nonsmokers. In: Journal of Immunology. 2008 ; Vol. 181, No. 8. pp. 5760-5767.
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abstract = "Lectins are innate immune defense proteins that recognize bacterial cell wall components. Based on the knowledge that cigarette smoking is associated with an increased risk of infections, we hypothesized that cigarette smoking may modulate the expression of lectin genes in airway epithelium. Affymetrix microarrays were used to survey the expression of lectin genes in large airway epithelium from nine nonsmokers and 20 healthy smokers and in small airway epithelium from 13 nonsmokers and 20 healthy smokers. There were no changes (>2-fold change; p < 0.05) in lectin gene expression among healthy smokers compared with nonsmokers except for down-regulation of intelectin 1, a lectin that binds to galactofuranosyl residues in bacterial cell walls (large airway epithelium, p < 0.01; small airway epithelium, p < 0.01). This was confirmed by TaqMan RT-PCR in both large (p < 0.05) and small airway epithelium (p < 0.02). Immunohistochemistry assessment of airway biopsies demonstrated that intelectin 1 was expressed in secretory cells, while Western analysis confirmed the decreased expression of intelectin 1 in airway epithelium of healthy smokers compared with healthy nonsmokers (p < 0.02). Finally, compared with healthy nonsmokers, intelectin 1 expression was also decreased in small airway epithelium of smokers with lone emphysema and normal spirometry (n = 13, p < 0.01) and smokers with established chronic obstructive pulmonary disease (n = 14, p < 0.01). In the context that intelectin 1 plays a role in defense against bacteria, its down-regulation in response to cigarette smoking is another example of the immunomodulatory effects of smoking on the immune system and may contribute to the increase in susceptibility to infections observed in smokers.",
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