Decreased α-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson's disease

Takahiko Tokuda, Sultan A. Salem, David Allsop, Toshiki Mizuno, Masanori Nakagawa, Mohamed M. Qureshi, Joseph J. Locascio, Michael G. Schlossmacher, Omar Ali El-Agnaf

Research output: Contribution to journalArticle

308 Citations (Scopus)

Abstract

There is ample biochemical, pathological, and genetic evidence that the metabolism of α-synuclein (α-syn) plays a crucial role in the pathogenesis of Parkinson disease (PD). To examine whether quantification of α-syn in cerebrospinal fluid (CSF) is potentially informative in the diagnosis of PD, we developed a specific ELISA system and measured the concentration of α-syn in CSF from 33 patients with PD (diagnosed according to UK PD Society Brain Bank criteria) and 38 control subjects including 9 neurologically healthy individuals. We found that PD patients had significantly lower α-syn levels in their CSF than the control groups (p < 0.0001) even after adjusting for gender and age. Age was independently associated with lower α-syn levels. Logistic regression analysis showed that reduction in CSF α-syn served as a significant predictor of PD beyond age and gender alone (area under ROC curve, c = 0.882). Furthermore, we observed a close inverse correlation between α-syn levels in CSF and assigned Hoehn and Yahr score in this cohort of 71 living subjects (p < 0.0001), even after adjusting for age. These findings identify in the quantification of α-syn from CSF a potential laboratory marker to aid the clinical diagnosis of PD.

Original languageEnglish
Pages (from-to)162-166
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume349
Issue number1
DOIs
Publication statusPublished - 13 Oct 2006
Externally publishedYes

Fingerprint

Synucleins
Cerebrospinal fluid
Parkinson Disease
Cerebrospinal Fluid
Metabolism
Regression analysis
ROC Curve
Area Under Curve
Logistics
Molecular Biology
Brain
Biomarkers
Logistic Models
Enzyme-Linked Immunosorbent Assay
Regression Analysis
Control Groups

Keywords

  • α-Synuclein
  • Biomarker
  • Cerebrospinal fluid
  • Diagnosis
  • ELISA
  • Neurodegenerative diseases
  • Parkinson disease

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Decreased α-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson's disease. / Tokuda, Takahiko; Salem, Sultan A.; Allsop, David; Mizuno, Toshiki; Nakagawa, Masanori; Qureshi, Mohamed M.; Locascio, Joseph J.; Schlossmacher, Michael G.; Ali El-Agnaf, Omar.

In: Biochemical and Biophysical Research Communications, Vol. 349, No. 1, 13.10.2006, p. 162-166.

Research output: Contribution to journalArticle

Tokuda, T, Salem, SA, Allsop, D, Mizuno, T, Nakagawa, M, Qureshi, MM, Locascio, JJ, Schlossmacher, MG & Ali El-Agnaf, O 2006, 'Decreased α-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson's disease', Biochemical and Biophysical Research Communications, vol. 349, no. 1, pp. 162-166. https://doi.org/10.1016/j.bbrc.2006.08.024
Tokuda, Takahiko ; Salem, Sultan A. ; Allsop, David ; Mizuno, Toshiki ; Nakagawa, Masanori ; Qureshi, Mohamed M. ; Locascio, Joseph J. ; Schlossmacher, Michael G. ; Ali El-Agnaf, Omar. / Decreased α-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson's disease. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 349, No. 1. pp. 162-166.
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