Deciphering the molecular machinery of stem cells: A look at the neoblast gene expression profile

Leonardo Rossi, Alessandra Salvetti, Francesco M. Marincola, Annalisa Lena, Paolo Deri, Linda Mannini, Renata Batistoni, Ena Wang, Vittorio Gremigni

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Background: Mammalian stem cells are difficult to access experimentally; model systems that can regenerate offer an alternative way to characterize stem cell related genes. Planarian regeneration depends on adult pluripotent stem cells - the neoblasts. These cells can be selectively destroyed using X-rays, enabling comparison of organisms lacking stem cells with wild-type worms. Results: Using a genomic approach we produced an oligonucleotide microarray chip (the Dj600 chip), which was designed using selected planarian gene sequences. Using this chip, we compared planarians treated with high doses of X-rays (which eliminates all neoblasts) with wild-type worms, which led to identification of a set of putatively neoblast-restricted genes. Most of these genes are involved in chromatin modeling and RNA metabolism, suggesting that epigenetic modifications and post-transcriptional regulation are pivotal in neoblast regulation. Comparing planarians treated with low doses of X-rays (after which some radiotolerant neoblasts re-populate the planarian body) with specimens irradiated with high doses and unirradiated control worms, we identified a group of genes that were upregulated as a consequence of low-dose X-ray treatment. Most of these genes encode proteins that are known to regulate the balance between death and survival of the cell; our results thus suggest that genetic programs that control neoblast cytoprotection, proliferation, and migration are activated by low-dose X-rays. Conclusion: The broad differentiation potential of planarian neoblasts is unparalleled by any adult stem cells in the animal kingdom. In addition to our validation of the Dj600 chip as a valuable platform, our work contributes to elucidating the molecular mechanisms that regulate the self-renewal and differentiation of neoblasts.

Original languageEnglish
Article numberR62
JournalGenome Biology
Volume8
Issue number4
DOIs
Publication statusPublished - 20 Apr 2007
Externally publishedYes

Fingerprint

Planarians
Transcriptome
machinery
gene expression
stem cells
X-radiation
Stem Cells
stem
X-Rays
gene
dosage
Adult Stem Cells
genes
Genes
Pluripotent Stem Cells
oligonucleotides
Chromatin Assembly and Disassembly
Cytoprotection
epigenetics
cell viability

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Rossi, L., Salvetti, A., Marincola, F. M., Lena, A., Deri, P., Mannini, L., ... Gremigni, V. (2007). Deciphering the molecular machinery of stem cells: A look at the neoblast gene expression profile. Genome Biology, 8(4), [R62]. https://doi.org/10.1186/gb-2007-8-4-r62

Deciphering the molecular machinery of stem cells : A look at the neoblast gene expression profile. / Rossi, Leonardo; Salvetti, Alessandra; Marincola, Francesco M.; Lena, Annalisa; Deri, Paolo; Mannini, Linda; Batistoni, Renata; Wang, Ena; Gremigni, Vittorio.

In: Genome Biology, Vol. 8, No. 4, R62, 20.04.2007.

Research output: Contribution to journalArticle

Rossi, L, Salvetti, A, Marincola, FM, Lena, A, Deri, P, Mannini, L, Batistoni, R, Wang, E & Gremigni, V 2007, 'Deciphering the molecular machinery of stem cells: A look at the neoblast gene expression profile', Genome Biology, vol. 8, no. 4, R62. https://doi.org/10.1186/gb-2007-8-4-r62
Rossi, Leonardo ; Salvetti, Alessandra ; Marincola, Francesco M. ; Lena, Annalisa ; Deri, Paolo ; Mannini, Linda ; Batistoni, Renata ; Wang, Ena ; Gremigni, Vittorio. / Deciphering the molecular machinery of stem cells : A look at the neoblast gene expression profile. In: Genome Biology. 2007 ; Vol. 8, No. 4.
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