Cytokine expression in human anterior pituitary adenomas

V. L. Green, Stephen Atkin, V. Speirs, R. V. Jeffreys, A. M. Landolt, B. Mathew, L. Hipkin, M. C. White

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Objective: There is increasing evidence for the role of cytokines in pituitary differentiated function and tumorigenesis, but the spectrum of cytokines found in the pituitary is unknown. Therefore profiles of cytokine expression were determined in different human anterior pituitary adenoma sub-types. Design: The reverse transcriptase-linked polymerase chain reaction (PCR) was used to identify the presence of cytokine mRNA within human pituitary adenomas. Patients: Seventeen pituitary adenoma biopsies removed at transsphenoidal surgery were examined: 4 somatotrophinomas, 7 non-functional adenomas, 4 prolactinomas, one case of Cushing's disease and one case of Nelson's syndrome. Measurements: RNA was extracted from each adenoma biopsy and reverse transcribed into cDNA. This was specifically amplified in a PCR using oligonucleotide primers complementary to each cytokine. The cytokines investigated were interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, tumour necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β 1 , β 2 and β 3. The products of each PCR were visualized using agarose gel electrophoresis. Results: All 17 adenomas expressed IL-8 transcripts, but no expression of IL-2, IL-5 or IL-7 was found. IL-6 was expressed in all 4 somatotrophinomas, 3 of 7 nonfunctional tumours, 2 of 4 prolactinomas and in the single case of Nelson's syndrome. At least one of the 3 isoforms of TGF-β was found in all but 2 tumours; one prolactinoma and one non-functional adenoma. IL-1α, IL-β, IL-4, TNF-α and TNF-β were expressed sporadically by individual adenomas. Conclusion: These data suggest that whilst IL-8 may be important, the local expression of the cytokines IL-2, IL-5 and IL-7 is not important in human anterior pituitary tumorigenesis.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalClinical Endocrinology
Volume45
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Pituitary Neoplasms
Cytokines
Adenoma
Prolactinoma
Interleukin-7
Interleukin-5
Nelson Syndrome
Tumor Necrosis Factor-alpha
Interleukin-8
Interleukin-1
Interleukin-2
Transforming Growth Factors
Interleukin-4
Interleukin-6
Carcinogenesis
Biopsy
Pituitary ACTH Hypersecretion
Polymerase Chain Reaction
DNA Primers
Agar Gel Electrophoresis

ASJC Scopus subject areas

  • Endocrinology

Cite this

Green, V. L., Atkin, S., Speirs, V., Jeffreys, R. V., Landolt, A. M., Mathew, B., ... White, M. C. (1996). Cytokine expression in human anterior pituitary adenomas. Clinical Endocrinology, 45(2), 179-185.

Cytokine expression in human anterior pituitary adenomas. / Green, V. L.; Atkin, Stephen; Speirs, V.; Jeffreys, R. V.; Landolt, A. M.; Mathew, B.; Hipkin, L.; White, M. C.

In: Clinical Endocrinology, Vol. 45, No. 2, 1996, p. 179-185.

Research output: Contribution to journalArticle

Green, VL, Atkin, S, Speirs, V, Jeffreys, RV, Landolt, AM, Mathew, B, Hipkin, L & White, MC 1996, 'Cytokine expression in human anterior pituitary adenomas', Clinical Endocrinology, vol. 45, no. 2, pp. 179-185.
Green VL, Atkin S, Speirs V, Jeffreys RV, Landolt AM, Mathew B et al. Cytokine expression in human anterior pituitary adenomas. Clinical Endocrinology. 1996;45(2):179-185.
Green, V. L. ; Atkin, Stephen ; Speirs, V. ; Jeffreys, R. V. ; Landolt, A. M. ; Mathew, B. ; Hipkin, L. ; White, M. C. / Cytokine expression in human anterior pituitary adenomas. In: Clinical Endocrinology. 1996 ; Vol. 45, No. 2. pp. 179-185.
@article{9db470dfb212412e8cef946e1e3a2471,
title = "Cytokine expression in human anterior pituitary adenomas",
abstract = "Objective: There is increasing evidence for the role of cytokines in pituitary differentiated function and tumorigenesis, but the spectrum of cytokines found in the pituitary is unknown. Therefore profiles of cytokine expression were determined in different human anterior pituitary adenoma sub-types. Design: The reverse transcriptase-linked polymerase chain reaction (PCR) was used to identify the presence of cytokine mRNA within human pituitary adenomas. Patients: Seventeen pituitary adenoma biopsies removed at transsphenoidal surgery were examined: 4 somatotrophinomas, 7 non-functional adenomas, 4 prolactinomas, one case of Cushing's disease and one case of Nelson's syndrome. Measurements: RNA was extracted from each adenoma biopsy and reverse transcribed into cDNA. This was specifically amplified in a PCR using oligonucleotide primers complementary to each cytokine. The cytokines investigated were interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, tumour necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β 1 , β 2 and β 3. The products of each PCR were visualized using agarose gel electrophoresis. Results: All 17 adenomas expressed IL-8 transcripts, but no expression of IL-2, IL-5 or IL-7 was found. IL-6 was expressed in all 4 somatotrophinomas, 3 of 7 nonfunctional tumours, 2 of 4 prolactinomas and in the single case of Nelson's syndrome. At least one of the 3 isoforms of TGF-β was found in all but 2 tumours; one prolactinoma and one non-functional adenoma. IL-1α, IL-β, IL-4, TNF-α and TNF-β were expressed sporadically by individual adenomas. Conclusion: These data suggest that whilst IL-8 may be important, the local expression of the cytokines IL-2, IL-5 and IL-7 is not important in human anterior pituitary tumorigenesis.",
author = "Green, {V. L.} and Stephen Atkin and V. Speirs and Jeffreys, {R. V.} and Landolt, {A. M.} and B. Mathew and L. Hipkin and White, {M. C.}",
year = "1996",
language = "English",
volume = "45",
pages = "179--185",
journal = "Clinical Endocrinology",
issn = "0300-0664",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Cytokine expression in human anterior pituitary adenomas

AU - Green, V. L.

AU - Atkin, Stephen

AU - Speirs, V.

AU - Jeffreys, R. V.

AU - Landolt, A. M.

AU - Mathew, B.

AU - Hipkin, L.

AU - White, M. C.

PY - 1996

Y1 - 1996

N2 - Objective: There is increasing evidence for the role of cytokines in pituitary differentiated function and tumorigenesis, but the spectrum of cytokines found in the pituitary is unknown. Therefore profiles of cytokine expression were determined in different human anterior pituitary adenoma sub-types. Design: The reverse transcriptase-linked polymerase chain reaction (PCR) was used to identify the presence of cytokine mRNA within human pituitary adenomas. Patients: Seventeen pituitary adenoma biopsies removed at transsphenoidal surgery were examined: 4 somatotrophinomas, 7 non-functional adenomas, 4 prolactinomas, one case of Cushing's disease and one case of Nelson's syndrome. Measurements: RNA was extracted from each adenoma biopsy and reverse transcribed into cDNA. This was specifically amplified in a PCR using oligonucleotide primers complementary to each cytokine. The cytokines investigated were interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, tumour necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β 1 , β 2 and β 3. The products of each PCR were visualized using agarose gel electrophoresis. Results: All 17 adenomas expressed IL-8 transcripts, but no expression of IL-2, IL-5 or IL-7 was found. IL-6 was expressed in all 4 somatotrophinomas, 3 of 7 nonfunctional tumours, 2 of 4 prolactinomas and in the single case of Nelson's syndrome. At least one of the 3 isoforms of TGF-β was found in all but 2 tumours; one prolactinoma and one non-functional adenoma. IL-1α, IL-β, IL-4, TNF-α and TNF-β were expressed sporadically by individual adenomas. Conclusion: These data suggest that whilst IL-8 may be important, the local expression of the cytokines IL-2, IL-5 and IL-7 is not important in human anterior pituitary tumorigenesis.

AB - Objective: There is increasing evidence for the role of cytokines in pituitary differentiated function and tumorigenesis, but the spectrum of cytokines found in the pituitary is unknown. Therefore profiles of cytokine expression were determined in different human anterior pituitary adenoma sub-types. Design: The reverse transcriptase-linked polymerase chain reaction (PCR) was used to identify the presence of cytokine mRNA within human pituitary adenomas. Patients: Seventeen pituitary adenoma biopsies removed at transsphenoidal surgery were examined: 4 somatotrophinomas, 7 non-functional adenomas, 4 prolactinomas, one case of Cushing's disease and one case of Nelson's syndrome. Measurements: RNA was extracted from each adenoma biopsy and reverse transcribed into cDNA. This was specifically amplified in a PCR using oligonucleotide primers complementary to each cytokine. The cytokines investigated were interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, tumour necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β 1 , β 2 and β 3. The products of each PCR were visualized using agarose gel electrophoresis. Results: All 17 adenomas expressed IL-8 transcripts, but no expression of IL-2, IL-5 or IL-7 was found. IL-6 was expressed in all 4 somatotrophinomas, 3 of 7 nonfunctional tumours, 2 of 4 prolactinomas and in the single case of Nelson's syndrome. At least one of the 3 isoforms of TGF-β was found in all but 2 tumours; one prolactinoma and one non-functional adenoma. IL-1α, IL-β, IL-4, TNF-α and TNF-β were expressed sporadically by individual adenomas. Conclusion: These data suggest that whilst IL-8 may be important, the local expression of the cytokines IL-2, IL-5 and IL-7 is not important in human anterior pituitary tumorigenesis.

UR - http://www.scopus.com/inward/record.url?scp=0029818280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029818280&partnerID=8YFLogxK

M3 - Article

C2 - 8881450

AN - SCOPUS:0029818280

VL - 45

SP - 179

EP - 185

JO - Clinical Endocrinology

JF - Clinical Endocrinology

SN - 0300-0664

IS - 2

ER -