Cyclic GMP-dependent and cyclic GMP-independent actions of nitric oxide on the renal afferent arteriole

Greg Trottier, Christopher Triggle, Sean K. O'Neill, Rodger Loutzenhiser

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

1. The effects of exogenous NO and endothelial-derived NO (EDNO) on the afferent arteriole were investigated in the in vitro perfused hydronephrotic rat kidney. Vessels were pre-constricted with angiotensin II (0.1-0.3 nM) or KCl (30 mM). NO was infused directly into the renal artery at concentrations ranging from 30-9000 nM. ODQ (10, 30 μM) was administered to examine the effects of guanylyl cyclase inhibition. Kidneys were treated with ibuprofen (10 μM) to avoid actions of prostaglandins. 2. During angiotensin II-induced vasoconstriction, NO elicited vasodilation at concentrations of 30-900 nM (EC 50 = 200 nM) and ODQ caused a 10 fold shift in NO-sensitivity (EC 50 1600 nM). During KCl-induced vasoconstriction, NO elicited a maximal dilation of 82 ± 9% at 9000 nM (EC 50 2000 nM) and ODQ had no effect. Thus in the presence of ODQ, the NO concentration-response curves for KCl- and angiotensin II-induced vasoconstriction were identical (P > 0.2). 3. To assess the possible role of cyclic GMP-independent mechanisms in the actions of EDNO, we compared the effects of L-NAME, ODQ and ODQ + L-NAME on acetylcholine-induced vasodilation. Angiotensin II reduced afferent arteriolar diameters from 16.7 ± 0.5 to 8.1 ± 0.8 microns and acetylcholine fully reversed this effect (16.9 ± 0.5 microns). ODQ restored the angiotensin II response in the presence of acetylcholine (7.1 ± 0.6 microns) and the subsequent addition of L-NAME had no further effect (6.8 ± 0.7 microns). Similarly, L-NAME alone, fully reversed the actions of acetylcholine. 4. Our findings indicate that exogenous NO is capable of eliciting renal afferent arteriolar vasodilation through both cyclic GMP-dependent and cyclic GMP-independent mechanisms. The cyclic GMP-independent action of NO did not require K + channel activation, as it could be elicited in the presence of 30 mM KCl. Finally, although cyclic GMP-independent effects of exogenous NO could be demonstrated in our model, EDNO appears to act exclusively through cyclic GMP.

Original languageEnglish
Pages (from-to)563-569
Number of pages7
JournalBritish Journal of Pharmacology
Volume125
Issue number3
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Cyclic GMP
Arterioles
Nitric Oxide
Angiotensin II
NG-Nitroarginine Methyl Ester
Kidney
Acetylcholine
Vasoconstriction
Vasodilation
Guanylate Cyclase
Ibuprofen
Renal Artery
Prostaglandins
Dilatation

Keywords

  • Acetylcholine
  • Afferent arteriole
  • Angiotensin II
  • Cyclic GMP
  • Hydronephrosis
  • KCl
  • Nitric oxide
  • Renal microcirculation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cyclic GMP-dependent and cyclic GMP-independent actions of nitric oxide on the renal afferent arteriole. / Trottier, Greg; Triggle, Christopher; O'Neill, Sean K.; Loutzenhiser, Rodger.

In: British Journal of Pharmacology, Vol. 125, No. 3, 1998, p. 563-569.

Research output: Contribution to journalArticle

Trottier, Greg ; Triggle, Christopher ; O'Neill, Sean K. ; Loutzenhiser, Rodger. / Cyclic GMP-dependent and cyclic GMP-independent actions of nitric oxide on the renal afferent arteriole. In: British Journal of Pharmacology. 1998 ; Vol. 125, No. 3. pp. 563-569.
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AU - Trottier, Greg

AU - Triggle, Christopher

AU - O'Neill, Sean K.

AU - Loutzenhiser, Rodger

PY - 1998

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N2 - 1. The effects of exogenous NO and endothelial-derived NO (EDNO) on the afferent arteriole were investigated in the in vitro perfused hydronephrotic rat kidney. Vessels were pre-constricted with angiotensin II (0.1-0.3 nM) or KCl (30 mM). NO was infused directly into the renal artery at concentrations ranging from 30-9000 nM. ODQ (10, 30 μM) was administered to examine the effects of guanylyl cyclase inhibition. Kidneys were treated with ibuprofen (10 μM) to avoid actions of prostaglandins. 2. During angiotensin II-induced vasoconstriction, NO elicited vasodilation at concentrations of 30-900 nM (EC 50 = 200 nM) and ODQ caused a 10 fold shift in NO-sensitivity (EC 50 1600 nM). During KCl-induced vasoconstriction, NO elicited a maximal dilation of 82 ± 9% at 9000 nM (EC 50 2000 nM) and ODQ had no effect. Thus in the presence of ODQ, the NO concentration-response curves for KCl- and angiotensin II-induced vasoconstriction were identical (P > 0.2). 3. To assess the possible role of cyclic GMP-independent mechanisms in the actions of EDNO, we compared the effects of L-NAME, ODQ and ODQ + L-NAME on acetylcholine-induced vasodilation. Angiotensin II reduced afferent arteriolar diameters from 16.7 ± 0.5 to 8.1 ± 0.8 microns and acetylcholine fully reversed this effect (16.9 ± 0.5 microns). ODQ restored the angiotensin II response in the presence of acetylcholine (7.1 ± 0.6 microns) and the subsequent addition of L-NAME had no further effect (6.8 ± 0.7 microns). Similarly, L-NAME alone, fully reversed the actions of acetylcholine. 4. Our findings indicate that exogenous NO is capable of eliciting renal afferent arteriolar vasodilation through both cyclic GMP-dependent and cyclic GMP-independent mechanisms. The cyclic GMP-independent action of NO did not require K + channel activation, as it could be elicited in the presence of 30 mM KCl. Finally, although cyclic GMP-independent effects of exogenous NO could be demonstrated in our model, EDNO appears to act exclusively through cyclic GMP.

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KW - Nitric oxide

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