Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin-induced type 1 diabetic rats

Vivian Soetikno, Flori R. Sari, Vijayakumar Sukumaran, Arun Lakshmanan, Meilei Harima, Kenji Suzuki, Hiroshi Kawachi, Kenichi Watanabe

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Diabetic kidney disease has been associated with the presence of lipid deposits. We assumed that curcumin, a polyphenol, would attenuate the tissue dyslipidemic condition through activation of 5' adenosine monophosphate (AMP)-activated protein kinase (AMPK) phosphorylation and suppression of sterol regulatory element-binding protein (SREBP)-1c in the kidney and would prevent renal progression in experimental type 1 diabetic rats. Diabetes was induced with streptozotocin (STZ) (55 mg/kg) by intraperitoneal injection in male Sprague-Dawley rats. Three weeks after STZ injection, rats were divided into three groups, namely, control, diabetic and diabetic treated with curcumin (100 mg/kg/day) by gavage for 8 weeks. We found that curcumin decreased plasma triglyceride and the amount of renal triglyceride significantly. Furthermore, treatment of diabetic rats with curcumin increased the phosphorylation of AMPK and prevented the increased renal expression of SREBP-1c and, as a result, decreased the expression of acetyl CoA carboxylase and fatty acid synthase as well as adipose differentiation-related protein, a marker of cytoplasmic droplets. We also demonstrate that curcumin significantly suppressed the increased expression of transforming growth factor β, vascular endothelial growth factor and extracellular matrix proteins such as type IV collagen and fibronectin. In addition, curcumin treatment increased nephrin expression to near-normal levels in diabetic rats. These results demonstrated that curcumin protects against the development of diabetic nephropathy through the AMPK-SREBP pathway and the reduction of renal triglyceride accumulation which could be a possible mechanism by which curcumin preserves renal function in diabetes.

Original languageEnglish
Pages (from-to)796-802
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume24
Issue number5
DOIs
Publication statusPublished - 1 May 2013
Externally publishedYes

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Sterol Regulatory Element Binding Proteins
AMP-Activated Protein Kinases
Curcumin
Streptozocin
Rats
Triglycerides
Kidney
Sterol Regulatory Element Binding Protein 1
Phosphorylation
Diabetic Nephropathies
Medical problems
Acetyl-CoA Carboxylase
Fatty Acid Synthases
Collagen Type IV
Extracellular Matrix Proteins
Transforming Growth Factors
Polyphenols
Adenosine Monophosphate
Intraperitoneal Injections
Fibronectins

Keywords

  • AMPK
  • Curcumin
  • Diabetic nephropathy
  • Dyslipidemia
  • SREBP
  • Streptozotocin-induced diabetes

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin-induced type 1 diabetic rats. / Soetikno, Vivian; Sari, Flori R.; Sukumaran, Vijayakumar; Lakshmanan, Arun; Harima, Meilei; Suzuki, Kenji; Kawachi, Hiroshi; Watanabe, Kenichi.

In: Journal of Nutritional Biochemistry, Vol. 24, No. 5, 01.05.2013, p. 796-802.

Research output: Contribution to journalArticle

Soetikno, Vivian ; Sari, Flori R. ; Sukumaran, Vijayakumar ; Lakshmanan, Arun ; Harima, Meilei ; Suzuki, Kenji ; Kawachi, Hiroshi ; Watanabe, Kenichi. / Curcumin decreases renal triglyceride accumulation through AMPK-SREBP signaling pathway in streptozotocin-induced type 1 diabetic rats. In: Journal of Nutritional Biochemistry. 2013 ; Vol. 24, No. 5. pp. 796-802.
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