Curcumin attenuates hyperglycaemia-mediated AMPK activation and oxidative stress in cerebrum of streptozotocin-induced diabetic rat

Arun Lakshmanan, Kenichi Watanabe, Rajarajan A. Thandavarayan, Flori R. Sari, Harima Meilei, Vivian Soetikno, Somasundaram Arumugam, Vijayasree V. Giridharan, Kenji Suzuki, Makoto Kodama

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Oxidative stress has been strongly implicated in the pathogenesis of diabetic encephalopathy (DE). Numerous studies have demonstrated a close relationship between oxidative stress and AMPK activation in various disorders, including diabetes-related brain disorders. Since curcumin has powerful antioxidant properties, this study investigated its effects on hyperglycaemia-mediated oxidative stress and AMPK activation in rats with DE. Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ-55 mg/kg BW). The diabetic rats were then orally administered curcumin (100 mg/kg BW) or vehicle for 8 weeks. The cerebra of the diabetic rats displayed upregulated protein expression of AdipoR1, p-AMPKα1, Tak1, GLUT4, NADPH oxidase sub-units, caspase-12 and 3-NT and increased lipid peroxidation in comparison with the controls and all of these effects were significantly attenuated with curcumin treatment, except for the increase in AdipoR1 expressions. These results provide a new insight into the beneficial effects of curcumin on hyperglycaemia-mediated DE, which are produced through the down-regulation of AMPK-mediated gluconeogenesis associated with its anti-oxidant property.

Original languageEnglish
Pages (from-to)788-795
Number of pages8
JournalFree Radical Research
Volume45
Issue number7
DOIs
Publication statusPublished - 1 Jul 2011
Externally publishedYes

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AMP-Activated Protein Kinases
Curcumin
Oxidative stress
Cerebrum
Brain Diseases
Streptozocin
Hyperglycemia
Rats
Oxidative Stress
Chemical activation
Medical problems
Caspase 12
Gluconeogenesis
NADPH Oxidase
Intraperitoneal Injections
Oxidants
Caspase 3
Lipid Peroxidation
Brain
Down-Regulation

Keywords

  • AMPK
  • apoptosis
  • Curcumin
  • diabetic encephalopathy
  • oxidative stress

ASJC Scopus subject areas

  • Biochemistry

Cite this

Curcumin attenuates hyperglycaemia-mediated AMPK activation and oxidative stress in cerebrum of streptozotocin-induced diabetic rat. / Lakshmanan, Arun; Watanabe, Kenichi; Thandavarayan, Rajarajan A.; Sari, Flori R.; Meilei, Harima; Soetikno, Vivian; Arumugam, Somasundaram; Giridharan, Vijayasree V.; Suzuki, Kenji; Kodama, Makoto.

In: Free Radical Research, Vol. 45, No. 7, 01.07.2011, p. 788-795.

Research output: Contribution to journalArticle

Lakshmanan, A, Watanabe, K, Thandavarayan, RA, Sari, FR, Meilei, H, Soetikno, V, Arumugam, S, Giridharan, VV, Suzuki, K & Kodama, M 2011, 'Curcumin attenuates hyperglycaemia-mediated AMPK activation and oxidative stress in cerebrum of streptozotocin-induced diabetic rat', Free Radical Research, vol. 45, no. 7, pp. 788-795. https://doi.org/10.3109/10715762.2011.579121
Lakshmanan, Arun ; Watanabe, Kenichi ; Thandavarayan, Rajarajan A. ; Sari, Flori R. ; Meilei, Harima ; Soetikno, Vivian ; Arumugam, Somasundaram ; Giridharan, Vijayasree V. ; Suzuki, Kenji ; Kodama, Makoto. / Curcumin attenuates hyperglycaemia-mediated AMPK activation and oxidative stress in cerebrum of streptozotocin-induced diabetic rat. In: Free Radical Research. 2011 ; Vol. 45, No. 7. pp. 788-795.
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