Curcumin attenuates hyperglycaemia-mediated AMPK activation and oxidative stress in cerebrum of streptozotocin-induced diabetic rat

Arun Prasath Lakshmanan, Kenichi Watanabe, Rajarajan A. Thandavarayan, Flori R. Sari, Harima Meilei, Vivian Soetikno, Somasundaram Arumugam, Vijayasree V. Giridharan, Kenji Suzuki, Makoto Kodama

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Abstract

Oxidative stress has been strongly implicated in the pathogenesis of diabetic encephalopathy (DE). Numerous studies have demonstrated a close relationship between oxidative stress and AMPK activation in various disorders, including diabetes-related brain disorders. Since curcumin has powerful antioxidant properties, this study investigated its effects on hyperglycaemia-mediated oxidative stress and AMPK activation in rats with DE. Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ-55 mg/kg BW). The diabetic rats were then orally administered curcumin (100 mg/kg BW) or vehicle for 8 weeks. The cerebra of the diabetic rats displayed upregulated protein expression of AdipoR1, p-AMPKα1, Tak1, GLUT4, NADPH oxidase sub-units, caspase-12 and 3-NT and increased lipid peroxidation in comparison with the controls and all of these effects were significantly attenuated with curcumin treatment, except for the increase in AdipoR1 expressions. These results provide a new insight into the beneficial effects of curcumin on hyperglycaemia-mediated DE, which are produced through the down-regulation of AMPK-mediated gluconeogenesis associated with its anti-oxidant property.

Original languageEnglish
Pages (from-to)788-795
Number of pages8
JournalFree Radical Research
Volume45
Issue number7
DOIs
Publication statusPublished - 1 Jul 2011

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Keywords

  • AMPK
  • Curcumin
  • apoptosis
  • diabetic encephalopathy
  • oxidative stress

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lakshmanan, A. P., Watanabe, K., Thandavarayan, R. A., Sari, F. R., Meilei, H., Soetikno, V., Arumugam, S., Giridharan, V. V., Suzuki, K., & Kodama, M. (2011). Curcumin attenuates hyperglycaemia-mediated AMPK activation and oxidative stress in cerebrum of streptozotocin-induced diabetic rat. Free Radical Research, 45(7), 788-795. https://doi.org/10.3109/10715762.2011.579121