Curcumin ameliorates macrophage infiltration by inhibiting NF-B activation and proinflammatory cytokines in streptozotocin induced-diabetic nephropathy

Vivian Soetikno, Flori R. Sari, Punniyakoti T. Veeraveedu, Rajarajan A. Thandavarayan, Meilei Harima, Vijayakumar Sukumaran, Arun Lakshmanan, Kenji Suzuki, Hiroshi Kawachi, Kenichi Watanabe

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Background: Chronic inflammation plays an important role in the progression of diabetic nephropathy (DN) and that the infiltration of macrophages in glomerulus has been implicated in the development of glomerular injury. We hypothesized that the plant polyphenolic compound curcumin, which is known to exert potent anti-inflammatory effect, would ameliorate macrophage infiltration in streptozotocin (STZ)-induced diabetic rats. Methods. Diabetes was induced with STZ (55 mg/kg) by intraperitoneal injection in rats. Three weeks after STZ injection, rats were divided into three groups, namely, control, diabetic, and diabetic treated with curcumin at 100 mg/kg/day, p.o., for 8 weeks. The rats were sacrificed 11 weeks after induction of diabetes. The excised kidney was used to assess macrophage infiltration and expression of various inflammatory markers. Results: At 11 weeks after STZ injection, diabetic rats exhibited renal dysfunction, as evidenced by reduced creatinine clearance, increased blood glucose, blood urea nitrogen and proteinuria, along with marked reduction in the body weight. All of these abnormalities were significantly reversed by curcumin. Hyperglycemia induced the degradation of IB and NF-B activation and as a result increased infiltration of macrophages (52%) as well as increased proinflammatory cytokines: TNF- and IL-1. Curcumin treatment significantly reduced macrophage infiltration in the kidneys of diabetic rats, suppressed the expression of above proinflammatory cytokines and degradation of IB. In addition, curcumin treatment also markedly decreased ICAM-1, MCP-1 and TGF- 1protein expression. Moreover, at nuclear level curcumin inhibited the NF-B activity. Conclusion: Our results suggested that curcumin treatment protect against the development of DN in rats by reducing macrophage infiltration through the inhibition of NF-B activation in STZ-induced diabetic rats.

Original languageEnglish
Article number35
JournalNutrition and Metabolism
Volume8
DOIs
Publication statusPublished - 14 Jun 2011
Externally publishedYes

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Curcumin
Diabetic Nephropathies
Streptozocin
Macrophages
Cytokines
Kidney
Injections
Blood Urea Nitrogen
Intercellular Adhesion Molecule-1
Intraperitoneal Injections
Interleukin-1
Proteinuria
Hyperglycemia
Blood Glucose
Creatinine
Anti-Inflammatory Agents
Therapeutics
Body Weight
Inflammation
Control Groups

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Endocrinology, Diabetes and Metabolism

Cite this

Curcumin ameliorates macrophage infiltration by inhibiting NF-B activation and proinflammatory cytokines in streptozotocin induced-diabetic nephropathy. / Soetikno, Vivian; Sari, Flori R.; Veeraveedu, Punniyakoti T.; Thandavarayan, Rajarajan A.; Harima, Meilei; Sukumaran, Vijayakumar; Lakshmanan, Arun; Suzuki, Kenji; Kawachi, Hiroshi; Watanabe, Kenichi.

In: Nutrition and Metabolism, Vol. 8, 35, 14.06.2011.

Research output: Contribution to journalArticle

Soetikno, Vivian ; Sari, Flori R. ; Veeraveedu, Punniyakoti T. ; Thandavarayan, Rajarajan A. ; Harima, Meilei ; Sukumaran, Vijayakumar ; Lakshmanan, Arun ; Suzuki, Kenji ; Kawachi, Hiroshi ; Watanabe, Kenichi. / Curcumin ameliorates macrophage infiltration by inhibiting NF-B activation and proinflammatory cytokines in streptozotocin induced-diabetic nephropathy. In: Nutrition and Metabolism. 2011 ; Vol. 8.
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T1 - Curcumin ameliorates macrophage infiltration by inhibiting NF-B activation and proinflammatory cytokines in streptozotocin induced-diabetic nephropathy

AU - Soetikno, Vivian

AU - Sari, Flori R.

AU - Veeraveedu, Punniyakoti T.

AU - Thandavarayan, Rajarajan A.

AU - Harima, Meilei

AU - Sukumaran, Vijayakumar

AU - Lakshmanan, Arun

AU - Suzuki, Kenji

AU - Kawachi, Hiroshi

AU - Watanabe, Kenichi

PY - 2011/6/14

Y1 - 2011/6/14

N2 - Background: Chronic inflammation plays an important role in the progression of diabetic nephropathy (DN) and that the infiltration of macrophages in glomerulus has been implicated in the development of glomerular injury. We hypothesized that the plant polyphenolic compound curcumin, which is known to exert potent anti-inflammatory effect, would ameliorate macrophage infiltration in streptozotocin (STZ)-induced diabetic rats. Methods. Diabetes was induced with STZ (55 mg/kg) by intraperitoneal injection in rats. Three weeks after STZ injection, rats were divided into three groups, namely, control, diabetic, and diabetic treated with curcumin at 100 mg/kg/day, p.o., for 8 weeks. The rats were sacrificed 11 weeks after induction of diabetes. The excised kidney was used to assess macrophage infiltration and expression of various inflammatory markers. Results: At 11 weeks after STZ injection, diabetic rats exhibited renal dysfunction, as evidenced by reduced creatinine clearance, increased blood glucose, blood urea nitrogen and proteinuria, along with marked reduction in the body weight. All of these abnormalities were significantly reversed by curcumin. Hyperglycemia induced the degradation of IB and NF-B activation and as a result increased infiltration of macrophages (52%) as well as increased proinflammatory cytokines: TNF- and IL-1. Curcumin treatment significantly reduced macrophage infiltration in the kidneys of diabetic rats, suppressed the expression of above proinflammatory cytokines and degradation of IB. In addition, curcumin treatment also markedly decreased ICAM-1, MCP-1 and TGF- 1protein expression. Moreover, at nuclear level curcumin inhibited the NF-B activity. Conclusion: Our results suggested that curcumin treatment protect against the development of DN in rats by reducing macrophage infiltration through the inhibition of NF-B activation in STZ-induced diabetic rats.

AB - Background: Chronic inflammation plays an important role in the progression of diabetic nephropathy (DN) and that the infiltration of macrophages in glomerulus has been implicated in the development of glomerular injury. We hypothesized that the plant polyphenolic compound curcumin, which is known to exert potent anti-inflammatory effect, would ameliorate macrophage infiltration in streptozotocin (STZ)-induced diabetic rats. Methods. Diabetes was induced with STZ (55 mg/kg) by intraperitoneal injection in rats. Three weeks after STZ injection, rats were divided into three groups, namely, control, diabetic, and diabetic treated with curcumin at 100 mg/kg/day, p.o., for 8 weeks. The rats were sacrificed 11 weeks after induction of diabetes. The excised kidney was used to assess macrophage infiltration and expression of various inflammatory markers. Results: At 11 weeks after STZ injection, diabetic rats exhibited renal dysfunction, as evidenced by reduced creatinine clearance, increased blood glucose, blood urea nitrogen and proteinuria, along with marked reduction in the body weight. All of these abnormalities were significantly reversed by curcumin. Hyperglycemia induced the degradation of IB and NF-B activation and as a result increased infiltration of macrophages (52%) as well as increased proinflammatory cytokines: TNF- and IL-1. Curcumin treatment significantly reduced macrophage infiltration in the kidneys of diabetic rats, suppressed the expression of above proinflammatory cytokines and degradation of IB. In addition, curcumin treatment also markedly decreased ICAM-1, MCP-1 and TGF- 1protein expression. Moreover, at nuclear level curcumin inhibited the NF-B activity. Conclusion: Our results suggested that curcumin treatment protect against the development of DN in rats by reducing macrophage infiltration through the inhibition of NF-B activation in STZ-induced diabetic rats.

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