Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats

M. T. Clunes, A. Collett, D. L. Baines, D. L. Bovell, H. Murphie, S. K. Inglis, H. L. McAlroy, R. E. Olver, S. M. Wilson

Research output: Contribution to journalArticle

Abstract

ATP and UTP did not evoke [Ca2+](i) signals in rat foetal lung epithelial cells grown on glass but elicited clear responses in cells grown into functionally polarised epithelia on permeable supports. Moreover, P2Y2 receptor mRNA could not be detected in cells on glass by the polymerase chain reaction but this mRNA species was clearly expressed by polarised cells. P2Y2 receptor expression thus appears to be a feature of the polarised phenotype.

Original languageEnglish
Pages (from-to)845-848
Number of pages4
JournalBritish Journal of Pharmacology
Volume124
Issue number5
DOIs
Publication statusPublished - 1 Jan 1998

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Keywords

  • Apical membrane
  • Epithelial cells
  • Epithelial polarity
  • Lung
  • Nucleotide receptors
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Pharmacology

Cite this

Clunes, M. T., Collett, A., Baines, D. L., Bovell, D. L., Murphie, H., Inglis, S. K., McAlroy, H. L., Olver, R. E., & Wilson, S. M. (1998). Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats. British Journal of Pharmacology, 124(5), 845-848. https://doi.org/10.1038/sj.bjp.0701942