Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats

M. T. Clunes, A. Collett, D. L. Baines, Douglas Bovell, H. Murphie, S. K. Inglis, H. L. McAlroy, R. E. Olver, S. M. Wilson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

ATP and UTP did not evoke [Ca2+](i) signals in rat foetal lung epithelial cells grown on glass but elicited clear responses in cells grown into functionally polarised epithelia on permeable supports. Moreover, P2Y2 receptor mRNA could not be detected in cells on glass by the polymerase chain reaction but this mRNA species was clearly expressed by polarised cells. P2Y2 receptor expression thus appears to be a feature of the polarised phenotype.

Original languageEnglish
Pages (from-to)845-847
Number of pages3
JournalBritish Journal of Pharmacology
Volume124
Issue number5
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Purinergic P2Y2 Receptors
Epithelial Cells
Lung
Glass
Messenger RNA
Uridine Triphosphate
Epithelium
Adenosine Triphosphate
Phenotype
Polymerase Chain Reaction

Keywords

  • Apical membrane
  • Epithelial cells
  • Epithelial polarity
  • Lung
  • Nucleotide receptors
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Pharmacology

Cite this

Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats. / Clunes, M. T.; Collett, A.; Baines, D. L.; Bovell, Douglas; Murphie, H.; Inglis, S. K.; McAlroy, H. L.; Olver, R. E.; Wilson, S. M.

In: British Journal of Pharmacology, Vol. 124, No. 5, 1998, p. 845-847.

Research output: Contribution to journalArticle

Clunes, MT, Collett, A, Baines, DL, Bovell, D, Murphie, H, Inglis, SK, McAlroy, HL, Olver, RE & Wilson, SM 1998, 'Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats', British Journal of Pharmacology, vol. 124, no. 5, pp. 845-847. https://doi.org/10.1038/sj.bjp.0701942
Clunes, M. T. ; Collett, A. ; Baines, D. L. ; Bovell, Douglas ; Murphie, H. ; Inglis, S. K. ; McAlroy, H. L. ; Olver, R. E. ; Wilson, S. M. / Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats. In: British Journal of Pharmacology. 1998 ; Vol. 124, No. 5. pp. 845-847.
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AU - Collett, A.

AU - Baines, D. L.

AU - Bovell, Douglas

AU - Murphie, H.

AU - Inglis, S. K.

AU - McAlroy, H. L.

AU - Olver, R. E.

AU - Wilson, S. M.

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KW - Epithelial polarity

KW - Lung

KW - Nucleotide receptors

KW - Polymerase chain reaction

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