Crystallographic and cryo EM analysis of virion-receptor interactions.

M. G. Rossmann, N. H. Olson, Prasanna Kolatkar, M. A. Oliveira, R. H. Cheng, J. M. Greve, A. McClelland, T. S. Baker

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cryoelectron microscopy has been used to determine the first structure of a virus when complexed with its glycoprotein cellular receptor. Human rhinovirus 16 (HRV16) complexed with the two amino-terminal, immunoglobulin-like domains of the intercellular adhesion molecule-1 (ICAM-1) shows that ICAM-1 binds into the 12 A deep "canyon" on the surface of the virus. This is consistent with the prediction that the viral receptor attachment site lies in a cavity inaccessible to the host's antibodies. The atomic structures of HRV14 and CD4, homologous to HRV16 and ICAM-1, showed excellent correspondence with observed density, thus establishing the virus-receptor interactions.

Original languageEnglish
Pages (from-to)531-541
Number of pages11
JournalArchives of virology. Supplementum
Volume9
Publication statusPublished - 1 Jan 1994
Externally publishedYes

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Intercellular Adhesion Molecule-1
Virion
Rhinovirus
Virus Receptors
Cryoelectron Microscopy
Viral Structures
Glycoproteins
Viruses
Antibodies

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Rossmann, M. G., Olson, N. H., Kolatkar, P., Oliveira, M. A., Cheng, R. H., Greve, J. M., ... Baker, T. S. (1994). Crystallographic and cryo EM analysis of virion-receptor interactions. Archives of virology. Supplementum, 9, 531-541.

Crystallographic and cryo EM analysis of virion-receptor interactions. / Rossmann, M. G.; Olson, N. H.; Kolatkar, Prasanna; Oliveira, M. A.; Cheng, R. H.; Greve, J. M.; McClelland, A.; Baker, T. S.

In: Archives of virology. Supplementum, Vol. 9, 01.01.1994, p. 531-541.

Research output: Contribution to journalArticle

Rossmann, MG, Olson, NH, Kolatkar, P, Oliveira, MA, Cheng, RH, Greve, JM, McClelland, A & Baker, TS 1994, 'Crystallographic and cryo EM analysis of virion-receptor interactions.', Archives of virology. Supplementum, vol. 9, pp. 531-541.
Rossmann MG, Olson NH, Kolatkar P, Oliveira MA, Cheng RH, Greve JM et al. Crystallographic and cryo EM analysis of virion-receptor interactions. Archives of virology. Supplementum. 1994 Jan 1;9:531-541.
Rossmann, M. G. ; Olson, N. H. ; Kolatkar, Prasanna ; Oliveira, M. A. ; Cheng, R. H. ; Greve, J. M. ; McClelland, A. ; Baker, T. S. / Crystallographic and cryo EM analysis of virion-receptor interactions. In: Archives of virology. Supplementum. 1994 ; Vol. 9. pp. 531-541.
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