Crystallization of sheep (Ovis aries) and goat (Capra hircus) haemoglobins under unbuffered low-salt conditions

K. Neelagandan, Pon Sathya Moorthy, Balasubramanian Moovarkumudalvan, M. N. Ponnuswamy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Haemoglobin is a tetrameric protein that plays a vital role in the transport of oxygen from the lungs to the tissues and of carbon dioxide back to the lungs. Even though a large amount of work has already been performed in this area, the study of the haemoglobin structures of avian and mammalian species is rather incomplete. Efforts are being made to understand the salient features of the species mentioned above. Here, whole blood plasma was collected from sheep and goat and purified by anion-exchange chromatography; the haemoglobins were crystallized by the hanging-drop vapour-diffusion method under unbuffered low-salt conditions using PEG 3350 as a precipitant. Data collection was carried out using a MAR345 image-plate detector system. Sheep haemoglobin crystallizes in the orthorhombic space group P212121 with one whole biological molecule (α2β2) in the asymmetric unit, with unit-cell parameters a = 60.231, b = 70.695, c = 131.479 Å. In contrast, goat haemoglobin crystallizes in the triclinic system with two biological molecules (α2β2) in the unit cell. The unit-cell parameters are a = 53.103, b = 69.382, c = 96.098 Å, α = 110.867, β = 91.133, γ = 109.437°.

Original languageEnglish
Pages (from-to)887-889
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number10
DOIs
Publication statusPublished - 19 Sep 2007
Externally publishedYes

Fingerprint

Domestic Sheep
goats
sheep
hemoglobin
Crystallization
Goats
Sheep
Hemoglobins
Salts
crystallization
salts
lungs
cells
blood plasma
Lung
Molecules
chromatography
Chromatography
Carbon Dioxide
Anions

Keywords

  • Haemoglobin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Crystallization of sheep (Ovis aries) and goat (Capra hircus) haemoglobins under unbuffered low-salt conditions. / Neelagandan, K.; Moorthy, Pon Sathya; Moovarkumudalvan, Balasubramanian; Ponnuswamy, M. N.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 63, No. 10, 19.09.2007, p. 887-889.

Research output: Contribution to journalArticle

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