Crystallization and preliminary X-ray crystallographic studies on the fungal immunomodulatory protein Fve from the golden needle mushroom (Flammulina velutipes)

See Voon Seow, I. Chun Kuo, Palasingam Paaventhan, Prasanna Kolatkar, Kaw Yan Chua

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The fungal immunomodulatory protein from the edible golden needle mushroom (Flammulina velutipes), designated Fve, is a single polypeptide consisting of 114 amino-acid residues. It is believed to trigger the mitogenic proliferation of T lymphocytes and Th1 cytokine production. Here, it is demonstrated that Fve forms a homodimer in nature. In order to understand the relationship between its structure and function, Fve was crystallized using the hanging-drop method; the protein formed well diffracting crystals within 3-5 d in 2.5% PEG 400, 2.0 M ammonium sulfate and 0.1 M Tris base buffer pH 8.5. The space group of the Fve crystals is either P43212 or P412 12, with unit-cell parameters a =- b = 96.92, c = 61.42 Å. The crystal contains two molecules per asymmetric unit and diffracts to 1.4 Å resolution when exposed to synchrotron radiation.

Original languageEnglish
Pages (from-to)1487-1489
Number of pages3
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number8
DOIs
Publication statusPublished - 1 Aug 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

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