Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL

Jeffrey A. Speir, Ussama M. Abdel-Motal, Mikael Jondal, Ian A. Wilson

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

T cell receptor (TCR) recognition of nonpeptidic and modified peptide antigens has been recently uncovered but is still poorly understood. Immunization with an H-2Kb-restricted glycopeptide RGY8-6H-Gal2 generates a population of cytotoxic T cells that express both α/β TCR, specific for glycopeptide, and γ/δ TCR, specific for the disaccharide, even on glycolipids. The crystal structure of Kb/RGY8-6H-Gal2 now demonstrates that the peptide and H-2Kb structures are unaffected by the peptide glycosylation, but the central region of the putative TCR binding site is dominated by the extensive exposure of the tethered carbohydrate. These features of the Kb/RGY8-6H-Gal2 structure are consistent with the individual ligand binding preferences identified for the α/β and γ/δ TCRs and thus explain the generation of a carbohydrate-specific T cell response.

Original languageEnglish
Pages (from-to)51-61
Number of pages11
JournalImmunity
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1999
Externally publishedYes

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Glycopeptides
T-Cell Antigen Receptor
Carbohydrates
Peptides
T-Lymphocytes
Disaccharides
Glycolipids
Glycosylation
Immunization
Binding Sites
Ligands
Antigens
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL. / Speir, Jeffrey A.; Abdel-Motal, Ussama M.; Jondal, Mikael; Wilson, Ian A.

In: Immunity, Vol. 10, No. 1, 01.1999, p. 51-61.

Research output: Contribution to journalArticle

Speir, Jeffrey A. ; Abdel-Motal, Ussama M. ; Jondal, Mikael ; Wilson, Ian A. / Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL. In: Immunity. 1999 ; Vol. 10, No. 1. pp. 51-61.
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