Crosslinking proteins to nucleic acids by ultraviolet laser irradiation

Illya G. Pashev, Stephan I. Dimitrov, Dimitar Angelov

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Ultraviolet (UV) irradiation can initiate complex formation between proteins and DNA or RNA and so can be used to study such interactions. However, crosslink formation by standard UV light sources can take up to several hours. More recently, a beam of monochromatic UV light from a laser has been used to initiate crosslinking in nano- and picosecond time intervals. As noted in an earlier TIBS article 'the advantages of short pulse times and high-energy fluxes should make this a valuable technique in the future'1. In this review we characterize laser-induced crosslinking and explore the applications of the method.

Original languageEnglish
Pages (from-to)323-326
Number of pages4
JournalTrends in Biochemical Sciences
Volume16
Issue numberC
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Ultraviolet lasers
Laser beam effects
Ultraviolet Rays
Crosslinking
Nucleic Acids
Lasers
Light sources
Proteins
Irradiation
RNA
Fluxes
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Crosslinking proteins to nucleic acids by ultraviolet laser irradiation. / Pashev, Illya G.; Dimitrov, Stephan I.; Angelov, Dimitar.

In: Trends in Biochemical Sciences, Vol. 16, No. C, 1991, p. 323-326.

Research output: Contribution to journalArticle

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