Covalent RGD modification of the inner pore surface of polycaprolactone scaffolds

Matthias Gabriel, Kamran Nazmi, Manfred Dahm, Andrej Zentner, Christian Friedrich Vahl, Dennis Strand

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Scaffold production for tissue engineering was demonstrated by means of a hot compression molding technique and subsequent particulate leaching. The utilization of spherical salt particles as the pore-forming agent ensured complete interconnectivity of the porous structure. This method obviated the use of potentially toxic organic solvents. To overcome the inherent non-cell-adhesive properties of the hydrophobic polymer polycaprolactone (PCL) surface activation with a diamine was performed, followed by the covalent immobilization of the adhesion-promoting RGD-peptide. The wet-chemical approach was performed to guarantee modification throughout the entire scaffold structure. The treatment was characterized by means of chemical and physical methods with respect to an exclusive surface modification without altering the bulk properties of the polymer. RGD-modified scaffolds were tested in cell-culture experiments to investigate the initial attachment and the proliferation of three different cell types.

Original languageEnglish
Pages (from-to)941-953
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume23
Issue number7
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Polycaprolactone
Scaffolds (biology)
Polymers
Diamines
Compression molding
Poisons
Hot pressing
Tissue Engineering
Tissue engineering
Cell culture
Scaffolds
Immobilization
Adhesives
Organic solvents
Peptides
Leaching
Surface treatment
Adhesion
Cell Culture Techniques
Salts

Keywords

  • BIOMIMETIC MATERIAL
  • CONFOCAL MICROSCOPY
  • POLYCAPROLACTONE
  • RGD PEPTIDE
  • SCAFFOLD
  • SURFACE MODIFICATION

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Covalent RGD modification of the inner pore surface of polycaprolactone scaffolds. / Gabriel, Matthias; Nazmi, Kamran; Dahm, Manfred; Zentner, Andrej; Vahl, Christian Friedrich; Strand, Dennis.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 23, No. 7, 2012, p. 941-953.

Research output: Contribution to journalArticle

Gabriel, Matthias ; Nazmi, Kamran ; Dahm, Manfred ; Zentner, Andrej ; Vahl, Christian Friedrich ; Strand, Dennis. / Covalent RGD modification of the inner pore surface of polycaprolactone scaffolds. In: Journal of Biomaterials Science, Polymer Edition. 2012 ; Vol. 23, No. 7. pp. 941-953.
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