Corneal Confocal Microscopy detects a Reduction in Corneal Endothelial Cells and Nerve Fibres in Patients with Acute Ischemic Stroke

Adnan Khan, Saadat Kamran, Naveed Akhtar, Georgios Ponirakis, Hamad Al-Muhannadi, Ioannis N. Petropoulos, Shumoos Al-Fahdawi, Rami Qahwaji, Faheem Sartaj, Blessy Babu, Muhammad Faisal Wadiwala, Ashfaq Shuaib, Rayaz Malik

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Abstract

Endothelial dysfunction and damage underlie cerebrovascular disease and ischemic stroke. We undertook corneal confocal microscopy (CCM) to quantify corneal endothelial cell and nerve morphology in 146 patients with an acute ischemic stroke and 18 age-matched healthy control participants. Corneal endothelial cell density was lower (P < 0.001) and endothelial cell area (P < 0.001) and perimeter (P < 0.001) were higher, whilst corneal nerve fibre density (P < 0.001), corneal nerve branch density (P < 0.001) and corneal nerve fibre length (P = 0.001) were lower in patients with acute ischemic stroke compared to controls. Corneal endothelial cell density, cell area and cell perimeter correlated with corneal nerve fiber density (P = 0.033, P = 0.014, P = 0.011) and length (P = 0.017, P = 0.013, P = 0.008), respectively. Multiple linear regression analysis showed a significant independent association between corneal endothelial cell density, area and perimeter with acute ischemic stroke and triglycerides. CCM is a rapid non-invasive ophthalmic imaging technique, which could be used to identify patients at risk of acute ischemic stroke.

Original languageEnglish
Article number17333
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

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Nerve Fibers
Confocal Microscopy
Endothelial Cells
Stroke
Cell Count
Cerebrovascular Disorders
Linear Models
Healthy Volunteers
Triglycerides
Regression Analysis

ASJC Scopus subject areas

  • General

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Corneal Confocal Microscopy detects a Reduction in Corneal Endothelial Cells and Nerve Fibres in Patients with Acute Ischemic Stroke. / Khan, Adnan; Kamran, Saadat; Akhtar, Naveed; Ponirakis, Georgios; Al-Muhannadi, Hamad; Petropoulos, Ioannis N.; Al-Fahdawi, Shumoos; Qahwaji, Rami; Sartaj, Faheem; Babu, Blessy; Wadiwala, Muhammad Faisal; Shuaib, Ashfaq; Malik, Rayaz.

In: Scientific Reports, Vol. 8, No. 1, 17333, 01.12.2018.

Research output: Contribution to journalArticle

Khan, A, Kamran, S, Akhtar, N, Ponirakis, G, Al-Muhannadi, H, Petropoulos, IN, Al-Fahdawi, S, Qahwaji, R, Sartaj, F, Babu, B, Wadiwala, MF, Shuaib, A & Malik, R 2018, 'Corneal Confocal Microscopy detects a Reduction in Corneal Endothelial Cells and Nerve Fibres in Patients with Acute Ischemic Stroke', Scientific Reports, vol. 8, no. 1, 17333. https://doi.org/10.1038/s41598-018-35298-3
Khan, Adnan ; Kamran, Saadat ; Akhtar, Naveed ; Ponirakis, Georgios ; Al-Muhannadi, Hamad ; Petropoulos, Ioannis N. ; Al-Fahdawi, Shumoos ; Qahwaji, Rami ; Sartaj, Faheem ; Babu, Blessy ; Wadiwala, Muhammad Faisal ; Shuaib, Ashfaq ; Malik, Rayaz. / Corneal Confocal Microscopy detects a Reduction in Corneal Endothelial Cells and Nerve Fibres in Patients with Acute Ischemic Stroke. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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