Corneal confocal microscopy

A non-invasive surrogate of nerve fibre damage and repair in diabetic patients

Rayaz Malik, P. Kallinikos, C. A. Abbott, C. H M Van Schie, P. Morgan, N. Efron, A. J M Boulton

Research output: Contribution to journalArticle

279 Citations (Scopus)

Abstract

Aims/hypothesis. The accurate detection, characterization and quantification of human diabetic neuropathy are important to define at risk patients, anticipate deterioration, and assess new therapies. Corneal confocal microscopy is a reiterative, rapid, non-invasive in vivo clinical examination technique capable of imaging corneal nerve fibres. The aim of this study was to define the ability of this technique to quantify the extent of degeneration and regeneration of corneal nerve fibres in diabetic patients with increasing neuropathic severity. Methods. We scanned the cornea and collected images of Bowman's layer (containing a rich nerve plexus) from 18 diabetic patients and 18 age-matched control subjects. Results. Corneal nerve fibre density (F3=9.6, p<0.0001), length (F3=23.8, p<0.0001), and branch density (F3=13.9, p<0.0001) were reduced in diabetic patients compared with control subjects, with a tendency for greater reduction in these measures with increasing severity of neuropathy. Conclusion/Interpretation. Corneal confocal microscopy is a rapid, non-invasive in vivo clinical examination technique which accurately defines the extent of corneal nerve damage and repair and acts as a surrogate measure of somatic neuropathy in diabetic patients. It could represent an advance to define the severity of neuropathy and expedite assessment of therapeutic efficacy in clinical trials of human diabetic neuropathy.

Original languageEnglish
Pages (from-to)683-688
Number of pages6
JournalDiabetologia
Volume46
Issue number5
Publication statusPublished - 1 May 2003
Externally publishedYes

Fingerprint

Nerve Fibers
Confocal Microscopy
Diabetic Neuropathies
Cornea
Regeneration
Clinical Trials
Therapeutics

Keywords

  • Confocal microscope
  • Corneal nerves
  • Degeneration
  • Diabetic neuropathy
  • Regeneration
  • Surrogate

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Malik, R., Kallinikos, P., Abbott, C. A., Van Schie, C. H. M., Morgan, P., Efron, N., & Boulton, A. J. M. (2003). Corneal confocal microscopy: A non-invasive surrogate of nerve fibre damage and repair in diabetic patients. Diabetologia, 46(5), 683-688.

Corneal confocal microscopy : A non-invasive surrogate of nerve fibre damage and repair in diabetic patients. / Malik, Rayaz; Kallinikos, P.; Abbott, C. A.; Van Schie, C. H M; Morgan, P.; Efron, N.; Boulton, A. J M.

In: Diabetologia, Vol. 46, No. 5, 01.05.2003, p. 683-688.

Research output: Contribution to journalArticle

Malik, R, Kallinikos, P, Abbott, CA, Van Schie, CHM, Morgan, P, Efron, N & Boulton, AJM 2003, 'Corneal confocal microscopy: A non-invasive surrogate of nerve fibre damage and repair in diabetic patients', Diabetologia, vol. 46, no. 5, pp. 683-688.
Malik R, Kallinikos P, Abbott CA, Van Schie CHM, Morgan P, Efron N et al. Corneal confocal microscopy: A non-invasive surrogate of nerve fibre damage and repair in diabetic patients. Diabetologia. 2003 May 1;46(5):683-688.
Malik, Rayaz ; Kallinikos, P. ; Abbott, C. A. ; Van Schie, C. H M ; Morgan, P. ; Efron, N. ; Boulton, A. J M. / Corneal confocal microscopy : A non-invasive surrogate of nerve fibre damage and repair in diabetic patients. In: Diabetologia. 2003 ; Vol. 46, No. 5. pp. 683-688.
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