Copy number variation analysis of matched ovarian primary tumors and peritoneal metastasis

Joel Malek, Eliane Mery, Yasmin A. Mahmoud, Eman K. Al-Azwani, Laurence Roger, Ruby Huang, Eva Jouve, Raphael Lis, Jean Paul Thiery, Denis Querleu, Arash Rafii Tabrizi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Ovarian cancer is the most deadly gynecological cancer. The high rate of mortality is due to the large tumor burden with extensive metastatic lesion of the abdominal cavity. Despite initial chemosensitivity and improved surgical procedures, abdominal recurrence remains an issue and results in patients' poor prognosis. Transcriptomic and genetic studies have revealed significant genome pathologies in the primary tumors and yielded important information regarding carcinogenesis. There are, however, few studies on genetic alterations and their consequences in peritoneal metastatic tumors when compared to their matched ovarian primary tumors. We used high-density SNP arrays to investigate copy number variations in matched primary and metastatic ovarian cancer from 9 patients. Here we show that copy number variations acquired by ovarian tumors are significantly different between matched primary and metastatic tumors and these are likely due to different functional requirements. We show that these copy number variations clearly differentially affect specific pathways including the JAK/STAT and cytokine signaling pathways. While many have shown complex involvement of cytokines in the ovarian cancer environment we provide evidence that ovarian tumors have specific copy number variation differences in many of these genes.

Original languageEnglish
Article numbere28561
JournalPLoS One
Volume6
Issue number12
DOIs
Publication statusPublished - 14 Dec 2011

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ovarian neoplasms
metastasis
Tumors
Neoplasm Metastasis
neoplasms
Neoplasms
Ovarian Neoplasms
cytokines
Genes
abdominal cavity
Cytokines
transcriptomics
Abdominal Cavity
carcinogenesis
Pathology
prognosis
Tumor Burden
surgery
Single Nucleotide Polymorphism
Carcinogenesis

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Copy number variation analysis of matched ovarian primary tumors and peritoneal metastasis. / Malek, Joel; Mery, Eliane; Mahmoud, Yasmin A.; Al-Azwani, Eman K.; Roger, Laurence; Huang, Ruby; Jouve, Eva; Lis, Raphael; Thiery, Jean Paul; Querleu, Denis; Tabrizi, Arash Rafii.

In: PLoS One, Vol. 6, No. 12, e28561, 14.12.2011.

Research output: Contribution to journalArticle

Malek, J, Mery, E, Mahmoud, YA, Al-Azwani, EK, Roger, L, Huang, R, Jouve, E, Lis, R, Thiery, JP, Querleu, D & Tabrizi, AR 2011, 'Copy number variation analysis of matched ovarian primary tumors and peritoneal metastasis', PLoS One, vol. 6, no. 12, e28561. https://doi.org/10.1371/journal.pone.0028561
Malek, Joel ; Mery, Eliane ; Mahmoud, Yasmin A. ; Al-Azwani, Eman K. ; Roger, Laurence ; Huang, Ruby ; Jouve, Eva ; Lis, Raphael ; Thiery, Jean Paul ; Querleu, Denis ; Tabrizi, Arash Rafii. / Copy number variation analysis of matched ovarian primary tumors and peritoneal metastasis. In: PLoS One. 2011 ; Vol. 6, No. 12.
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