HpC1, a Charon 4A bacteriophage containing a 16.3-kilobase insert of human genomic DNA, has been identified as representing the 3' portion of the human α2(I) collagen gene by hybrid selected translation and cross-reactivity with characterized sheep and chick collagen genes. Evaluation of HpC1 by restriction endonuclease mapping and α2(I) mRNA hybridization demonstrated that the α2(I) mRNA coding regions are separated by noncoding regions including one of 2.7 kilobases pairs near the 3' end of the mRNA coding region. In addition, a 1.8-kilobase XbaI-BamHI fragment containing the 3' end of the α2(I)mRNA coding region includes a region of DNA that is repeated many times throughout the human genome. Quantification of α2(I) gene number, using the technique of DNA dot hybridization, with two ecoRI fragments from HpC1 as probes, demonstrated that HpC1 is represented once in the human genome. In this context, it is unlikely that human α2(I) collagen chain production is modulated by differential transcription of multiple α2(I) genes.
|Number of pages||7|
|Journal||Journal of Biological Chemistry|
|Publication status||Published - 1 Dec 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology