Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species

Maamoun Fatfat, Raghida A. Merhi, Omar Rahal, Detcho A. Stoyanovsky, Angela Zaki, Hazar Haidar, Valerian E. Kagan, Hala Gali-Muhtasib, Khaled Machaca

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Metals including iron, copper and zinc are essential for physiological processes yet can be toxic at high concentrations. However the role of these metals in the progression of cancer is not well defined. Here we study the anti-tumor activity of the metal chelator, TPEN, and define its mechanism of action.Methods: Multiple approaches were employed, including cell viability, cell cycle analysis, multiple measurements of apoptosis, and mitochondrial function. In addition we measured cellular metal contents and employed EPR to record redox cycling of TPEN-metal complexes. Mouse xenografts were also performed to test the efficacy of TPEN in vivo.Results: We show that metal chelation using TPEN (5μM) selectively induces cell death in HCT116 colon cancer cells without affecting the viability of non-cancerous colon or intestinal cells. Cell death was associated with increased levels of reactive oxygen species (ROS) and was inhibited by antioxidants and by prior chelation of copper. Interestingly, HCT116 cells accumulate copper to 7-folds higher levels than normal colon cells, and the TPEN-copper complex engages in redox cycling to generate hydroxyl radicals. Consistently, TPEN exhibits robust anti-tumor activity in vivo in colon cancer mouse xenografts.Conclusion: Our data show that TPEN induces cell death by chelating copper to produce TPEN-copper complexes that engage in redox cycling to selectively eliminate colon cancer cells.

Original languageEnglish
Article number527
JournalBMC Cancer
Volume14
Issue number1
DOIs
Publication statusPublished - 21 Jul 2014

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Colonic Neoplasms
Oxidation-Reduction
Copper
Reactive Oxygen Species
Metals
Cell Death
Heterografts
Colon
Physiological Phenomena
HCT116 Cells
Neoplasms
N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine
Poisons
Coordination Complexes
Chelating Agents
Hydroxyl Radical
Zinc
Cell Survival
Cell Cycle
Iron

Keywords

  • Colon cancer
  • Copper
  • Metal chelation
  • Reactive oxygen species
  • Redox cycling
  • TPEN

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

Cite this

Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species. / Fatfat, Maamoun; Merhi, Raghida A.; Rahal, Omar; Stoyanovsky, Detcho A.; Zaki, Angela; Haidar, Hazar; Kagan, Valerian E.; Gali-Muhtasib, Hala; Machaca, Khaled.

In: BMC Cancer, Vol. 14, No. 1, 527, 21.07.2014.

Research output: Contribution to journalArticle

Fatfat, M, Merhi, RA, Rahal, O, Stoyanovsky, DA, Zaki, A, Haidar, H, Kagan, VE, Gali-Muhtasib, H & Machaca, K 2014, 'Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species', BMC Cancer, vol. 14, no. 1, 527. https://doi.org/10.1186/1471-2407-14-527
Fatfat, Maamoun ; Merhi, Raghida A. ; Rahal, Omar ; Stoyanovsky, Detcho A. ; Zaki, Angela ; Haidar, Hazar ; Kagan, Valerian E. ; Gali-Muhtasib, Hala ; Machaca, Khaled. / Copper chelation selectively kills colon cancer cells through redox cycling and generation of reactive oxygen species. In: BMC Cancer. 2014 ; Vol. 14, No. 1.
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