Control of the histone-acetyltransferase activity of Tip60 by the HIV-1 transactivator protein, Tat

Martina Creaven, Fabienne Hans, Vesco Mutskov, Edwige Col, Cécile Caron, Stefan Dimitrov, Saadi Khochbin

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Tip60, a cellular histone-acetyltransferase, is known to interact with the HIV-1-encoded transactivator protein, Tat. In this work, we show that the interaction of Tat with Tip60 efficiently inhibits the Tip60 histone- acetyltransferase activity besides its histone-acetyltransferase activity, Tip60 can undergo an autoacetylation which is not affected by Tat interaction. Our data show that Tip60 does not significantly influence Tat- dependent transcriptional activation of the 5'-LTR of HIV, suggesting that its interaction with Tat affects some intrinsic cellular process. We were then able to identify a cellular gene, Mn-dependent superoxide dismutase (Mn- SOD), that has a Tip60-dependent transcriptional activity. Interestingly, the simultaneous expression of Tat and Tip60 abolishes the effect of Tip60 on the activity of the Mn-SOD promoter. We postulate that the HIV-1 transactivator, Tat, in targeting Tip60 hinders the expression of cellular genes (such as Mn- SOD) which normally interfere with the efficient replication and propagation of the virus.

Original languageEnglish
Pages (from-to)8826-8830
Number of pages5
JournalBiochemistry
Volume38
Issue number27
DOIs
Publication statusPublished - 6 Jul 1999
Externally publishedYes

Fingerprint

Human Immunodeficiency Virus rev Gene Products
tat Gene Products
Histone Acetyltransferases
Trans-Activators
Superoxide Dismutase
HIV-1
Genes
Virus Replication
Viruses
Transcriptional Activation
Chemical activation
HIV
Gene Expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Control of the histone-acetyltransferase activity of Tip60 by the HIV-1 transactivator protein, Tat. / Creaven, Martina; Hans, Fabienne; Mutskov, Vesco; Col, Edwige; Caron, Cécile; Dimitrov, Stefan; Khochbin, Saadi.

In: Biochemistry, Vol. 38, No. 27, 06.07.1999, p. 8826-8830.

Research output: Contribution to journalArticle

Creaven, M, Hans, F, Mutskov, V, Col, E, Caron, C, Dimitrov, S & Khochbin, S 1999, 'Control of the histone-acetyltransferase activity of Tip60 by the HIV-1 transactivator protein, Tat', Biochemistry, vol. 38, no. 27, pp. 8826-8830. https://doi.org/10.1021/bi9907274
Creaven, Martina ; Hans, Fabienne ; Mutskov, Vesco ; Col, Edwige ; Caron, Cécile ; Dimitrov, Stefan ; Khochbin, Saadi. / Control of the histone-acetyltransferase activity of Tip60 by the HIV-1 transactivator protein, Tat. In: Biochemistry. 1999 ; Vol. 38, No. 27. pp. 8826-8830.
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