Continuous biohydrogen production from starch wastewater via sequential dark-photo fermentation with emphasize on maghemite nanoparticles

Mahmoud Nasr, Ahmed Tawfik, Shinichi Ookawara, Masaaki Suzuki, Sheena Kumari, Faizal Bux

Research output: Contribution to journalArticle

35 Citations (Scopus)


Hydrogen production from starch wastewater via sequential dark-photo fermentation process was investigated. Two anaerobic baffled reactors (ABRs) were operated in parallel at an OLR of 8.11 ± 0.97g-COD/L/d, and a HRT of 15h. ABR-1 and ABR-2 was inoculated with pre-treated sludge and sludge immobilized on maghemite nanoparticles, respectively. Better hydrogen yield of 104.75 ± 12.39mL-H2/g-COD-removed was achieved in ABR-2 as compared to 66.22 ± 4.88mL-H2/g-COD-removed in ABR-1. The effluent of ABR-2 was used for further hydrogen production by photo fermentation in ABR-3. An overall hydrogen yield of 166.83 ± 27.79mL-H2/g-COD-removed was achieved at a total HRT of 30h. 16S rRNA phylogeny showed that Clostridium and Rhodopseudomonas palustris species were dominant in ABR-1, ABR-2 and ABR-3, respectively.

Original languageEnglish
Pages (from-to)500-506
Number of pages7
JournalJournal of Industrial and Engineering Chemistry
Publication statusPublished - 25 Jan 2015
Externally publishedYes



  • Anaerobic baffled reactor
  • Dark-photo fermentation
  • Hydrogen
  • Maghemite nanoparticles
  • Starch wastewater

ASJC Scopus subject areas

  • Chemical Engineering(all)

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