Construction and characterization of hexon-chimeric adenoviruses: Specification of adenovirus serotype

J. G D Gall, Ronald Crystal, E. Falck-Pedersen

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type- determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

Original languageEnglish
Pages (from-to)10260-10264
Number of pages5
JournalJournal of Virology
Volume72
Issue number12
Publication statusPublished - 25 Nov 1998
Externally publishedYes

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Adenoviridae
Epitopes
Genes
Viruses
Neutralizing Antibodies
Immune System
Serogroup

ASJC Scopus subject areas

  • Immunology

Cite this

Construction and characterization of hexon-chimeric adenoviruses : Specification of adenovirus serotype. / Gall, J. G D; Crystal, Ronald; Falck-Pedersen, E.

In: Journal of Virology, Vol. 72, No. 12, 25.11.1998, p. 10260-10264.

Research output: Contribution to journalArticle

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