Construction and characterization of hexon-chimeric adenoviruses: Specification of adenovirus serotype

J. G.D. Gall, R. G. Crystal, E. Falck-Pedersen

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Abstract

This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type- determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

Original languageEnglish
Pages (from-to)10260-10264
Number of pages5
JournalJournal of Virology
Volume72
Issue number12
Publication statusPublished - 25 Nov 1998

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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