Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization

Adrian Sprenger, Sebastian Weber, Mostafa Zarai, Rudolf Engelke, Juliana M. Nascimento, Christine Gretzmeier, Martin Hilpert, Melanie Boerries, Cristina Has, Hauke Busch, Leena Bruckner-Tuderman, Jörn Dengjel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Keratinocytes account for 95% of all cells of the epidermis, the stratified squamous epithelium forming the outer layer of the skin, in which a significant number of skin diseases takes root. Immortalized keratinocyte cell lines are often used as research model systems providing standardized, reproducible, and homogenous biological material. Apart from that, primary human keratinocytes are frequently used for medical studies because the skin provides an important route for drug administration and is readily accessible for biopsies. However, comparability of these cell systems is not known. Cell lines may undergo phenotypic shifts and may differ from the in vivo situation in important aspects. Primary cells, on the other hand, may vary in biological functions depending on gender and age of the donor and localization of the biopsy specimen. Here we employed metabolic labeling in combination with quantitative mass spectrometry-based proteomics to assess A431 and HaCaT cell lines for their suitability as model systems. Compared with cell lines, comprehensive profiling of the primary human keratinocyte proteome with respect to gender, age, and skin localization identified an unexpected high proteomic consistency. The data were analyzed by an improved ontology enrichment analysis workflow designed for the study of global proteomics experiments. It enables a quick, comprehensive and unbiased overview of altered biological phenomena and links experimental data to literature. We guide through our workflow, point out its advantages compared with other methods and apply it to visualize differences of cell lines compared with primary human keratinocytes.

Original languageEnglish
Pages (from-to)2509-2521
Number of pages13
JournalMolecular and Cellular Proteomics
Volume12
Issue number9
DOIs
Publication statusPublished - 1 Sep 2013
Externally publishedYes

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Proteome
Keratinocytes
Skin
Cells
Cell Line
Proteomics
Workflow
Biopsy
Drug Administration Routes
Biological Phenomena
Skin Diseases
Epidermis
Biological materials
Labeling
Mass spectrometry
Ontology
Mass Spectrometry
Epithelium
Research
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization. / Sprenger, Adrian; Weber, Sebastian; Zarai, Mostafa; Engelke, Rudolf; Nascimento, Juliana M.; Gretzmeier, Christine; Hilpert, Martin; Boerries, Melanie; Has, Cristina; Busch, Hauke; Bruckner-Tuderman, Leena; Dengjel, Jörn.

In: Molecular and Cellular Proteomics, Vol. 12, No. 9, 01.09.2013, p. 2509-2521.

Research output: Contribution to journalArticle

Sprenger, A, Weber, S, Zarai, M, Engelke, R, Nascimento, JM, Gretzmeier, C, Hilpert, M, Boerries, M, Has, C, Busch, H, Bruckner-Tuderman, L & Dengjel, J 2013, 'Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization', Molecular and Cellular Proteomics, vol. 12, no. 9, pp. 2509-2521. https://doi.org/10.1074/mcp.M112.025478
Sprenger, Adrian ; Weber, Sebastian ; Zarai, Mostafa ; Engelke, Rudolf ; Nascimento, Juliana M. ; Gretzmeier, Christine ; Hilpert, Martin ; Boerries, Melanie ; Has, Cristina ; Busch, Hauke ; Bruckner-Tuderman, Leena ; Dengjel, Jörn. / Consistency of the proteome in primary human keratinocytes with respect to gender, age, and skin localization. In: Molecular and Cellular Proteomics. 2013 ; Vol. 12, No. 9. pp. 2509-2521.
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