Conservation of immune gene signatures in solid tumors and prognostic implications

Julia Chifman, Ashok Pullikuth, Jeff W. Chou, Davide Bedognetti, Lance D. Miller

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Tumor-infiltrating leukocytes can either limit cancer growth or facilitate its spread. Diagnostic strategies that comprehensively assess the functional complexity of tumor immune infiltrates could have wide-reaching clinical value. In previous work we identified distinct immune gene signatures in breast tumors that reflect the relative abundance of infiltrating immune cells and exhibited significant associations with patient outcomes. Here we hypothesized that immune gene signatures agnostic to tumor type can be identified by de novo discovery of gene clusters enriched for immunological functions and possessing internal correlation structure conserved across solid tumors from different anatomic sites. Methods: We assembled microarray expression datasets encompassing 5,295 tumors of the breast, colon, lung, ovarian and prostate. Unsupervised clustering methods were used to determine number and composition of gene clusters within each dataset. Immune-enriched gene clusters (signatures) identified by gene ontology enrichment were analyzed for internal correlation structure and conservation across tumors then compared against expression profiles of: 1) flow-sorted leukocytes from peripheral blood and 2) >300 cancer cell lines from solid and hematologic cancers. Cox regression analysis was used to identify signatures with significant associations with clinical outcome. Results: We identified nine distinct immune-enriched gene signatures conserved across all five tumor types. The signatures differentiated specific leukocyte lineages with moderate discernment overall, and naturally organized into six discrete groups indicative of admixed lineages. Moreover, seven of the signatures exhibit minimal and uncorrelated expression in cancer cell lines, suggesting that these signatures derive predominantly from infiltrating immune cells. All nine immune signatures achieved statistically significant associations with patient prognosis (p<0.05) in one or more tumor types with greatest significance observed in breast and skin cancers. Several signatures indicative of myeloid lineages exhibited poor outcome associations that were most apparent in brain and colon cancers. Conclusions: These findings suggest that tumor infiltrating immune cells can be differentiated by immune-specific gene expression patterns that quantify the relative abundance of multiple immune infiltrates across a range of solid tumor types. That these markers of immune involvement are significantly associated with patient prognosis in diverse cancers suggests their clinical utility as pan-cancer markers of tumor behavior and immune responsiveness.

Original languageEnglish
Article number911
JournalBMC Cancer
Volume16
Issue number1
DOIs
Publication statusPublished - 22 Nov 2016

Fingerprint

Genes
Neoplasms
Multigene Family
Leukocytes
Breast Neoplasms
Biomarkers
Cell Line
Gene Ontology
Skin Neoplasms
Tumor Biomarkers
Brain Neoplasms
Colonic Neoplasms
Cluster Analysis
Prostate
Colon
Regression Analysis
Gene Expression
Lung
Growth

Keywords

  • Consensus clustering
  • Enrichment scores
  • Immune signatures
  • Survival analysis

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

Cite this

Conservation of immune gene signatures in solid tumors and prognostic implications. / Chifman, Julia; Pullikuth, Ashok; Chou, Jeff W.; Bedognetti, Davide; Miller, Lance D.

In: BMC Cancer, Vol. 16, No. 1, 911, 22.11.2016.

Research output: Contribution to journalArticle

Chifman, Julia ; Pullikuth, Ashok ; Chou, Jeff W. ; Bedognetti, Davide ; Miller, Lance D. / Conservation of immune gene signatures in solid tumors and prognostic implications. In: BMC Cancer. 2016 ; Vol. 16, No. 1.
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