Conformational flexibility of Mycobacterium tuberculosis thioredoxin reductase

Crystal structure and normal-mode analysis

Mohd Akif, Karsten Suhre, Chandra Verma, Shekhar C. Mande

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The thioredoxin system exists ubiquitously and participates in essential antioxidant and redox-regulation processes via a pair of conserved cysteine residues. In Mycobacterium tuberculosis, which lacks a genuine glutathione system, the thioredoxin system provides reducing equivalents inside the cell. The three-dimensional structure of thioredoxin reductase from M. tuberculosis has been determined at 3 Å resolution. TLS refinement reveals a large libration axis, showing that NADPH-binding domain has large anisotropic disorder. The relative rotation of the NADPH domain with respect to the FAD domain is necessary for the thioredoxin reduction cycle, as it brings the spatially distant reacting sites close together. Normal-mode analysis carried out based on the elastic network model shows that the motion required to bring about the functional conformational change can be accounted for by motion along one single mode. TLS refinement and normal-mode analysis thus enhance our understanding of the associated conformational changes.

Original languageEnglish
Pages (from-to)1603-1611
Number of pages9
JournalActa Crystallographica Section D: Biological Crystallography
Volume61
Issue number12
DOIs
Publication statusPublished - Dec 2005
Externally publishedYes

Fingerprint

Thioredoxin-Disulfide Reductase
tuberculosis
Thioredoxins
Mycobacterium tuberculosis
flexibility
Crystal structure
NADP
crystal structure
Flavin-Adenine Dinucleotide
glutathione
libration
cysteine
antioxidants
Oxidation-Reduction
Glutathione
Cysteine
Antioxidants
disorders
cycles
cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Conformational flexibility of Mycobacterium tuberculosis thioredoxin reductase : Crystal structure and normal-mode analysis. / Akif, Mohd; Suhre, Karsten; Verma, Chandra; Mande, Shekhar C.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 61, No. 12, 12.2005, p. 1603-1611.

Research output: Contribution to journalArticle

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