Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype

Andrea W. Guennoun, Melissa Kazantzis, Remy Thomas, Martin Wabitsch, Daniel Tews, Seetharama S. Konduru, Mouaadh Abdelkarim, Vladimir Zilberfarb, Donny A. Strosberg, Lotfi Chouchane

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Functional brown adipose tissue (BAT), involved in energy expenditure, has recently been detected in substantial amounts in adults. Formerly overlooked BAT has now become an attractive anti-obesity target. Methods and results: Molecular characterization of human brown and white adipocytes, using a myriad of techniques including high-throughput RNA sequencing and functional assays, showed that PAZ6 and SW872 cells exhibit classical molecular and phenotypic markers of brown and white adipocytes, respectively. However, the pre-adipocyte cell line SGBS presents a versatile phenotype. A transit expression of classical brown markers such as UCP1 and PPARγ peaked and declined at day 28 post-differentiation initiation. Conversely, white adipocyte markers, including Tcf21, showed reciprocal behavior. Interestingly, leptin levels peaked at day 28 whereas the highest adiponectin mRNA levels were detected at day 14 of differentiation. Phenotypic analysis of the abundance and shape of lipid droplets were consistent with the molecular patterns. Accordingly, the oxidative capacity of SGBS adipocytes peaked on differentiation day 14 and declined progressively towards differentiation day 28. Conclusions: Our studies have unveiled a new phenotype of human adipocytes, providing a tool to identify molecular gene expression patterns and pathways involved in the conversion between white and brown adipocytes.

Original languageEnglish
Article number135
JournalJournal of Translational Medicine
Volume13
Issue number1
DOIs
Publication statusPublished - 30 Apr 2015

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White Adipocytes
Brown Adipocytes
Adipocytes
Tissue
Phenotype
Peroxisome Proliferator-Activated Receptors
Brown Adipose Tissue
Adiponectin
Leptin
Gene expression
Assays
Cells
Throughput
RNA
Lipids
High-Throughput Nucleotide Sequencing
Messenger RNA
Energy Metabolism
Obesity
Gene Expression

Keywords

  • BAT
  • Beige adipocytes
  • Human adipocytes
  • Non-shivering thermogenesis
  • Obesity
  • SGBS cells
  • WAT

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype. / Guennoun, Andrea W.; Kazantzis, Melissa; Thomas, Remy; Wabitsch, Martin; Tews, Daniel; Konduru, Seetharama S.; Abdelkarim, Mouaadh; Zilberfarb, Vladimir; Strosberg, Donny A.; Chouchane, Lotfi.

In: Journal of Translational Medicine, Vol. 13, No. 1, 135, 30.04.2015.

Research output: Contribution to journalArticle

Guennoun, Andrea W. ; Kazantzis, Melissa ; Thomas, Remy ; Wabitsch, Martin ; Tews, Daniel ; Konduru, Seetharama S. ; Abdelkarim, Mouaadh ; Zilberfarb, Vladimir ; Strosberg, Donny A. ; Chouchane, Lotfi. / Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype. In: Journal of Translational Medicine. 2015 ; Vol. 13, No. 1.
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