Complementary techniques: RNA amplification for gene profiling analysis

Ena Wang, Monica Panelli, Francesco M. Marincola

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The study of clinical samples is often limited by the amount of material available. DNA and RNA can be amplified from small specimens and, therefore, used for high-throughput analyses. While precise estimates of the level of DNA concentration in a given specimen is rarely studied (with the exception of relatively crude analyses of gene amplification or loss in cancer specimens), it is critical to know the proportional expression of various RNA transcripts since this proportion governs cell function by modulating the expression of various proteins. In addition, accurate estimates of relative RNA expression in biological conditions portray the reaction of cells to environmental stimuli shedding light on the characteristics of the microenvironment associated with particular physiologic or pathologic conditions. For this reason, the development of technologies for high fidelity messenger RNA amplification have been focused of extreme interest in the past decade with specific aim not only of increasing the abundance of RNA available to study but to accurately maintain the proportionality of expression of various RNA species among each other within a given specimen. This chapter will discuss various approaches to proportional RNA amplification focusing on amplification of the whole transcriptome (all transcripts in a given samples) rather than individual genes. These methods are suitable for high-throughput transcriptional profiling studies.

Original languageEnglish
Title of host publicationMicroarray Technology and Cancer Gene Profiling
PublisherSpringer New York
Pages39-53
Number of pages15
Volume593
ISBN (Print)9780387399775
DOIs
Publication statusPublished - 2007
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume593
ISSN (Print)00652598

Fingerprint

Complementary RNA
Gene Amplification
Amplification
Genes
RNA
Throughput
DNA
Transcriptome
Technology
Messenger RNA
Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Wang, E., Panelli, M., & Marincola, F. M. (2007). Complementary techniques: RNA amplification for gene profiling analysis. In Microarray Technology and Cancer Gene Profiling (Vol. 593, pp. 39-53). (Advances in Experimental Medicine and Biology; Vol. 593). Springer New York. https://doi.org/10.1007/978-0-387-39978-2_5

Complementary techniques : RNA amplification for gene profiling analysis. / Wang, Ena; Panelli, Monica; Marincola, Francesco M.

Microarray Technology and Cancer Gene Profiling. Vol. 593 Springer New York, 2007. p. 39-53 (Advances in Experimental Medicine and Biology; Vol. 593).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wang, E, Panelli, M & Marincola, FM 2007, Complementary techniques: RNA amplification for gene profiling analysis. in Microarray Technology and Cancer Gene Profiling. vol. 593, Advances in Experimental Medicine and Biology, vol. 593, Springer New York, pp. 39-53. https://doi.org/10.1007/978-0-387-39978-2_5
Wang E, Panelli M, Marincola FM. Complementary techniques: RNA amplification for gene profiling analysis. In Microarray Technology and Cancer Gene Profiling. Vol. 593. Springer New York. 2007. p. 39-53. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-0-387-39978-2_5
Wang, Ena ; Panelli, Monica ; Marincola, Francesco M. / Complementary techniques : RNA amplification for gene profiling analysis. Microarray Technology and Cancer Gene Profiling. Vol. 593 Springer New York, 2007. pp. 39-53 (Advances in Experimental Medicine and Biology).
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