Comparative studies on peptides representing the so-called tachykinin-like region of the Alzheimer Aβ peptide [Aβ(25-35)]

Omar Ali El-Agnaf, G. Brent Irvine, Geraldine Fitzpatrick, W. Kenneth Glass, David J S Guthrie

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In an attempt to answer the question of whether or not the so-called tachykinin-like region of the Alzheimer β-amyloid protein [Aβ(25-35)] can act as a tachykinin, the sequences Aβ(25-35), Aβ(25-35)amide and their norleucine-35 and phenylalanine-31 analogues were synthesized. These peptides were examined with ligand binding studies, electron microscopy, CD and NMR. In all cases some differences were found between the Aβ(25-35) analogue and the corresponding Phe31 peptide. In addition, in ligand displacement studies on tachykinin NK1 receptors, only the Phe31 analogue showed activity comparable to that of genuine tachykinins. We conclude that peptides based on Aβ(25-35) but with a Phe residue at position 31 do display properties typical of a tachykinin, but that peptides with Ile at this position do not.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalBiochemical Journal
Volume336
Issue number2
Publication statusPublished - 1 Dec 1998
Externally publishedYes

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Tachykinins
Peptides
Norleucine
Tachykinin Receptors
Ligands
Serum Amyloid A Protein
Phenylalanine
Amides
Electron Microscopy
Electron microscopy
Nuclear magnetic resonance
peptide A

ASJC Scopus subject areas

  • Biochemistry

Cite this

Comparative studies on peptides representing the so-called tachykinin-like region of the Alzheimer Aβ peptide [Aβ(25-35)]. / Ali El-Agnaf, Omar; Irvine, G. Brent; Fitzpatrick, Geraldine; Glass, W. Kenneth; Guthrie, David J S.

In: Biochemical Journal, Vol. 336, No. 2, 01.12.1998, p. 419-427.

Research output: Contribution to journalArticle

Ali El-Agnaf, Omar ; Irvine, G. Brent ; Fitzpatrick, Geraldine ; Glass, W. Kenneth ; Guthrie, David J S. / Comparative studies on peptides representing the so-called tachykinin-like region of the Alzheimer Aβ peptide [Aβ(25-35)]. In: Biochemical Journal. 1998 ; Vol. 336, No. 2. pp. 419-427.
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