Comparative evaluation of hexokinase isoenzyme typing and antigen detection for differentiation of Entamoeba histolytica and Entamoeba dispar.

Devendra Bansal, Rakesh Sehgal, Yogesh Chawla, Ramesh Chander Mahajan, Nancy Malla

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Entamoeba histolytica, the causative organism of invasive amebiasis is a potential pathogen, while asymptomatic infection is caused by E. dispar. Differentiation of the species is not possible on the basis of morphological characters by microscopic examination. In the present study an attempt has been made to differentiate E. histolytica from E. dispar in 45 isolates obtained from culture and direct stool samples respectively on the basis of hexokinase isoenzyme analysis and Tech Lab ELISA. A 100% correlation was found between these two techniques. However, Tech Lab E. histolytica antigen detection test was found to be both rapid and technically simple. Its use in diagnosis and epidemiological studies is recommended.

Original languageEnglish
Pages (from-to)123-125
Number of pages3
JournalNepal Medical College journal : NMCJ
Volume6
Issue number2
Publication statusPublished - 1 Jan 2004
Externally publishedYes

Fingerprint

Entamoeba
Entamoeba histolytica
Hexokinase
Differentiation Antigens
Isoenzymes
Amebiasis
Asymptomatic Infections
Epidemiologic Studies
Enzyme-Linked Immunosorbent Assay
Antigens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Comparative evaluation of hexokinase isoenzyme typing and antigen detection for differentiation of Entamoeba histolytica and Entamoeba dispar. / Bansal, Devendra; Sehgal, Rakesh; Chawla, Yogesh; Mahajan, Ramesh Chander; Malla, Nancy.

In: Nepal Medical College journal : NMCJ, Vol. 6, No. 2, 01.01.2004, p. 123-125.

Research output: Contribution to journalArticle

@article{aea5c299c0a94386bad0434b0287b2e0,
title = "Comparative evaluation of hexokinase isoenzyme typing and antigen detection for differentiation of Entamoeba histolytica and Entamoeba dispar.",
abstract = "Entamoeba histolytica, the causative organism of invasive amebiasis is a potential pathogen, while asymptomatic infection is caused by E. dispar. Differentiation of the species is not possible on the basis of morphological characters by microscopic examination. In the present study an attempt has been made to differentiate E. histolytica from E. dispar in 45 isolates obtained from culture and direct stool samples respectively on the basis of hexokinase isoenzyme analysis and Tech Lab ELISA. A 100{\%} correlation was found between these two techniques. However, Tech Lab E. histolytica antigen detection test was found to be both rapid and technically simple. Its use in diagnosis and epidemiological studies is recommended.",
author = "Devendra Bansal and Rakesh Sehgal and Yogesh Chawla and Mahajan, {Ramesh Chander} and Nancy Malla",
year = "2004",
month = "1",
day = "1",
language = "English",
volume = "6",
pages = "123--125",
journal = "Nepal Medical College journal : NMCJ",
number = "2",

}

TY - JOUR

T1 - Comparative evaluation of hexokinase isoenzyme typing and antigen detection for differentiation of Entamoeba histolytica and Entamoeba dispar.

AU - Bansal, Devendra

AU - Sehgal, Rakesh

AU - Chawla, Yogesh

AU - Mahajan, Ramesh Chander

AU - Malla, Nancy

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Entamoeba histolytica, the causative organism of invasive amebiasis is a potential pathogen, while asymptomatic infection is caused by E. dispar. Differentiation of the species is not possible on the basis of morphological characters by microscopic examination. In the present study an attempt has been made to differentiate E. histolytica from E. dispar in 45 isolates obtained from culture and direct stool samples respectively on the basis of hexokinase isoenzyme analysis and Tech Lab ELISA. A 100% correlation was found between these two techniques. However, Tech Lab E. histolytica antigen detection test was found to be both rapid and technically simple. Its use in diagnosis and epidemiological studies is recommended.

AB - Entamoeba histolytica, the causative organism of invasive amebiasis is a potential pathogen, while asymptomatic infection is caused by E. dispar. Differentiation of the species is not possible on the basis of morphological characters by microscopic examination. In the present study an attempt has been made to differentiate E. histolytica from E. dispar in 45 isolates obtained from culture and direct stool samples respectively on the basis of hexokinase isoenzyme analysis and Tech Lab ELISA. A 100% correlation was found between these two techniques. However, Tech Lab E. histolytica antigen detection test was found to be both rapid and technically simple. Its use in diagnosis and epidemiological studies is recommended.

UR - http://www.scopus.com/inward/record.url?scp=33748569867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748569867&partnerID=8YFLogxK

M3 - Article

C2 - 16295743

AN - SCOPUS:33748569867

VL - 6

SP - 123

EP - 125

JO - Nepal Medical College journal : NMCJ

JF - Nepal Medical College journal : NMCJ

IS - 2

ER -